微生物絮凝剂产生菌HF-8的筛选与培养条件优化

Screening and the cultivating conditions optimized for microbial flocculant-producing bacteria(HF-8)

从污水处理厂的活性污泥中筛选出微生物絮凝剂HF-8,并对菌株的培养基组成和培养条件进行了优化;培养基最佳配方(g/500 mL):蔗糖10 g、(NH4)2SO40.2 g、KH2PO42 g、K2HPO42.5 g、NaCl0.05 g、Mg(SO4).7H2O0.1 g、水500 mL;最佳培养条件:温度30℃、初始pH值6.0、摇床转速120 r/min,优化之后的絮凝剂对高龄土悬浊液的絮凝率提高到了90.3%。

Microbial flocculant-producing bacteria （ HF-8 ） were screened from active sludge of wastewater treatment plant. To this stmin, s culture medium composition and the culture conditions were optimized. The culture medium best formula is （g/500ml） ： cane ： 10g, ammonium sulfate ： 0.2g, monopotassium phosphate ： 2g, dipotassium hydrogen phosphate：2.5g, sodium chloride ：0.05g, heptahydrate magnesium sulfate：0.1 g, water：500ml. The best culture condition is：the culture temperature ：30. C,the initial pH ：6,rotate speed of shack bed： 120r/min.