摘要
本文分别选用由木瓜蛋白酶水解天山马鹿茸血获得的肽Ⅰ、肽Ⅱ以及原血为受试样品,研究天山马鹿茸血及其酶解肽对小鼠免疫功能和抗氧化功能的影响,其中肽Ⅰ、肽ⅡDH分别为18.1%、12.2%时,清除OH.能力最强,清除率为50.8%。分别测定了脏器指数,脾细胞增殖实验,血清中溶血素和抗体生成细胞的含量以及小鼠血清总抗氧化能力、SOD活力和MDA含量。结果表明:与对照组相比较,肽Ⅱ组能极显著提高小鼠体液和细胞免疫功能,加强小鼠的抗氧化功能(P<0.01或P<0.05)。此外,具有较强抗氧化活性的肽显示出了很强的免疫活性(P<0.05)。
The effect of Cervus elaphus Velvet antler blood(CVAB) and its hydrolysates (peptide Ⅰ and Ⅱ) on immunological and anti-oxidative function of mice was investigated on this paper. The index of immune organs, proliferation of mice spleen lymphocyte in vitro, hemolysin content in serum, antibody-producing cell of spleen, anti-oxidation index, SOD activity and MDA content of serum in mice were carried out. Peptide Ⅱwith highest clearance rate of OH·(50%) obviously enhance immunological and anti-oxidative function of mice ( P 〈 0.01 or P 〈0. 05 ). There is a significant correlation between anti-oxidation and immunity (P 〈 0.05 ).
出处
《天然产物研究与开发》
CAS
CSCD
2009年第5期757-760,共4页
Natural Product Research and Development
基金
国家自然科学基金(30571347)
863项目(2007AA10Z325)
关键词
天山马鹿茸血
免疫
抗氧化
溶血素
总抗
SOD
Cervus elaphus Velvet antler blood
immunity
anti-oxidation
serum hemolysin
anti-oxidation index
SOD activity