马鹿茸血酶解肽体内免疫功能及抗氧化功能关系的研究

Effect of Peptides Hydrolyzed from Cervus elaphus Velvet Antler Blood on the Immunity and Anti-oxidation of Mice

本文分别选用由木瓜蛋白酶水解天山马鹿茸血获得的肽Ⅰ、肽Ⅱ以及原血为受试样品,研究天山马鹿茸血及其酶解肽对小鼠免疫功能和抗氧化功能的影响,其中肽Ⅰ、肽ⅡDH分别为18.1%、12.2%时,清除OH.能力最强,清除率为50.8%。分别测定了脏器指数,脾细胞增殖实验,血清中溶血素和抗体生成细胞的含量以及小鼠血清总抗氧化能力、SOD活力和MDA含量。结果表明:与对照组相比较,肽Ⅱ组能极显著提高小鼠体液和细胞免疫功能,加强小鼠的抗氧化功能(P<0.01或P<0.05)。此外,具有较强抗氧化活性的肽显示出了很强的免疫活性(P<0.05)。

The effect of Cervus elaphus Velvet antler blood（CVAB） and its hydrolysates （peptide Ⅰ and Ⅱ） on immunological and anti-oxidative function of mice was investigated on this paper. The index of immune organs, proliferation of mice spleen lymphocyte in vitro, hemolysin content in serum, antibody-producing cell of spleen, anti-oxidation index, SOD activity and MDA content of serum in mice were carried out. Peptide Ⅱwith highest clearance rate of OH·（50%） obviously enhance immunological and anti-oxidative function of mice （ P 〈 0.01 or P 〈0. 05 ）. There is a significant correlation between anti-oxidation and immunity （P 〈 0.05 ）.