红藻藻胆体内部蛋白间的能量传递研究 Ⅰ.人工合成R-PE/R-PC/APC复合物内的能量传递

Energy Transfer among Proteins in the Phycobilisome of Red Algae Ⅰ.Energy Transfer in Synthesized Complex of R PC/APC

研究了人工合成的藻胆体模拟复合物的时间分辨荧光光谱，并运用多指数拟合的方法对数据进行了详细的处理和分析，结果表明能量在Ｒ－ＰＥ和Ｒ－ＰＣ之间的传递时间与能量从Ｒ－ＰＣ传递到ＡＰＣ的时间几乎相等（５０ｐｓ）；除此之外，Ｒ－ＰＥ到ＡＰＣ还有两种能量传递的通道，能量在这两个通道的传递时间分别为１１０ｐｓ和４００ｐｓ．即：蛋白之间的能量传递通道是并行的．

Time resolved fluorescence spectra of artificial synthesized model of phycobilisome are studied in detail by multi exponential deconvolution technique.The results show that the energy transfer period between neighboring proteins is 50 ps, and there are another two path ways for energy transfer from R PE to APC with time constant of 110 ps and 400 ps These energy transfer path ways are parallel