GnRH对虎纹蛙LH和FSH分泌活动的调节作用

Regulation of GnRH on LH and FSH Secretion of Rana rugulosa

通过在体和离体实验，研究了ｍＧｎＲＨ和ｃＧｎＲＨ－Ⅱ对生殖前期虎纹蛙垂体ＬＨ及ＦＳＨ的合成与分泌的影响．结果表明：在体条件下，ｍＧｎＲＨ和ｃＧｎＲＨ－Ⅱ都能极显著刺激♀蛙和♂蛙垂体ＬＨ及ＦＳＨ的释放，也能显著升高♀蛙垂体ＬＨ的含量，但对♂蛙垂体ＬＨ及ＦＳＨ的含量无明显影响；ｃＧｎＲＨ－Ⅱ能极显著升高♀蛙垂体ＦＳＨ的含量．在刺激♀蛙垂体ＬＨ的合成、释放以及♂蛙垂体ＦＳＨ的释放方面，ｍＧｎＲＨ的作用显著强于ｃＧｎＲＨ－Ⅱ．离体实验中，０．１ｎｇ／ｍＬ的ｍＧｎＲＨ和１ｎｇ／ｍＬ的ｃＧｎＲＨ－Ⅱ都能极显著刺激离体♀蛙垂体碎片ＬＨ的释放．

The effects of mGnRH and cGnRH Ⅱ on the synthesis and secretion of LH and FSH of pituitary of Rana rugulosa during pre breeding stage were studied in vivo and in vitro. In vivo both mGnRH and cGnRH Ⅱ very significantly stimulated the release of LH and FSH from the pituitary of female and male. They also significantly elevated the LH levels of pituitary of female, but had no notable effects on LH or FSH content of pituitary of male. The FSH content of pituitary of female was also very significantly elevated by cGnRH Ⅱ. mGnRH was more effective than cGnRH Ⅱ in stimulating the LH synthesis and release of pituitary of female, or the FSH release of pituitary of male. In vitro both mGnRH at the dose of 0.1 ng/mL and cGnRH Ⅱ at the dose of 1 ng/mL very remarkably stimulated LH release from the incubated pituitary fragments of female.