基于CellSense生物传感器的垃圾渗滤液毒性分析

Toxicity Assessments of Landfill Leachate Based on CellSense Biosensor

采用基于大肠杆菌（E.coli）的CellSense生物传感器,对上海某垃圾填埋场渗滤液处理系统垃圾渗滤液原液（1号）、兼性塘（2号）、曝气氧化塘（3号）、矿化垃圾生物床（4号）和FeC内电解（5号）各处理单元的水质毒性进行跟踪分析.结果表明,在该垃圾渗滤液的处理过程中,由于渗滤液的基质作用和难降解有机物生成的毒性中间产物等的影响,处理工艺中部分单元的水质毒性出现升高现象.CellSense生物传感器分析得上述1~5号单元的水质综合毒性分别为90%（EC10）、80%（EC30）、60%（EC50）、80%（EC50）和2%（EC50）,毒性分析结果与对应的COD和氨氮等指标无明显相关性.

The toxicity of landfill leachate of domestic wastes in different treatment unites landfill leachate pretreatment （No. 1 ）, facultative lagoon （No. 2）, aerated lagoon （ No. 3 ）, mineralized refuse bioreactor （ No. 4 ） and catalysis iron inner electrolysis （ No. 5 ） of leachate treatment process in Shanghai was determined based on CellSense biosensor with E. coli. The results showed that the toxicity of the effluent in different treatment unites for landfill leachate increased during the treatment process due to the influence of matrix effect and intermediate toxic products of refractory organic matters. The toxicity of the effluent from No. 1 to No.5 unites are 90% （ECl0） ,80% （EC30） ,60% （EC50） ,80% （EC50） and 2% （EC50）, respectively. This showed no significant correlation with their corresponding COD and ammonia nitrogen concentrations.