黄毛耳草药材质量标准研究

Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC

目的:建立黄毛耳草药材的质量标准。方法:以乙腈-水(7∶93)为流动相,测定波长为238nm,采用HPLC测定黄毛耳草药材中鸡矢藤苷甲酯的含量。结果:鸡矢藤苷甲酯在22.08～552mg·L-1与峰面积呈良好线性关系,加样回收率为99.7%(n=6),RSD0.72%。结论:方法简便、快速,可作为黄毛耳草药材的质量标准。

Objective：To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha. Method：The determination was carried out on an HC-C18 column elnted with acetonitrile-water （7∶93） as mobile phase,and the detection wavelength was set at 238 nm. Result：There is a good linearity in the range 22.08-552 mg·L^-1 of scandoside methyl ester and the average recovery is 97.7% （n=6）,RSD 0.72%. Conclusion：This method is convenient,quick,and is applicable to the quality control of H. chrysotricha.