骨髓间充质干细胞和内皮祖细胞作为组织工程瓣种子细胞的对比

Mesenchymal stem cells and endothelial progenitor cells differentiate into endothelially seeded cells for tissue engineering of heart valve

目的诱导分化骨髓间充质干细胞（BMSC）及内皮祖细胞（EPC），进行BMSC及EPC作为组织工程瓣膜（TEHV）种子细胞的对比。方法分离扩增BMSC及EPC，分别定向诱导分化为内皮细胞，比较两种细胞在形态学、增殖能力、黏附力以及细胞冻存和复苏率方面的特点与区别。结果光镜下，两种细胞均为贴壁生长细胞，BMSC的形态为梭形或多角型，EPC的形态为圆形及不规则形状，第2代诱导的BMSCs、EPCs的倍增时间分别为34h和35h，诱导后的BMSC冻存和复苏率以及黏附力与EPC之间差异无统计学意义；电镜示两种细胞均能够种植在去细胞猪主动脉瓣上。免疫组织化学结果显示两种细胞种植后的瓣膜上均有间质细胞生长。结论BMSC和EPC诱导后可分化为内皮细胞，两者之间差异无统计学意义，都是合适的TEHV种子细胞。

Objective To differentiate mesenchymal stem cells （MSCs） and endothelial progenitor cells （ EPCs）, and compare them used as the seeded cells of tissue engineering heart valves. Methods The canine bone MSCs （BMSCs） and EPCs were separated, expanded and differentiated into endothelial cells. The induced BMSCs and EPCs were cultured, expanded, purified, frozen and resuscitated. The BMSC and EPC-derived endothelial cells were examined in cell morphology, proliferation capability, adhering capability and adhesion onto detergent acellularized porcine valves. The capability of cell resuscitation was studied between the two kinds of endothelial cells. Results Both BMSC and EPC-derived endothelial cells were adhesive growth cells, and spindle in shape. The double time of the BMSC- and EPC-derived endothelial cells was about 34 h and 35 h, respectively. There was no significant difference in adhering capability and cell resuscitating rate. Scanning microscopy revealed that the two kinds of cells could be planted on the detergent aeellularized porcine-valves. Conclusion BMSCs and EPCs can be induced to differentiate into endothelial cells, and can be used as seeded cells to fabricate tissue engineered heart valves.