摘要
目的观察小檗碱(Berberine,以下简称“Ber”)对结肠癌细胞株HT-29增殖、凋亡的作用并探讨其离子通道机制。方法Ber0.1—30.0μmol/L处理HT-29细胞。氮蓝四唑盐法检测细胞增殖,流式细胞术检测凋亡率,膜片钳检测延迟整流钾通道[IK(V)]。结果0.1~30.0μmol/L Ber均可抑制HT-29细胞增殖(P〈0.05),其抑癌率与作用时间及浓度相关;HT-29细胞经Ber处理24h后,凋亡率明显增高(P〈0.05),0.1、0.3、3.0、30.0μmol/LBer组凋亡率分别为(7.6±1.8)%、(9.8±2.1)%、(22.3±4.2)%、(40.6±4.5)%,对照组凋亡率为(2.4±1.1)%;0.3、3.0、30.0μmol/L的Ber能浓度依赖性抑制结肠癌细胞IK(V)(P〈0.01),阶跃刺激为+80mV时,对照组、0.3、3.0、30.0μmol/LBer组IK(V)分别为(488±42)、(376±22)、(296±25)、(225±34)pA,0.3、3.0、30.0μmol/LBer处理组IK(V)分别为对照组的(77.16±5.41)%、(61.35±6.09)%、(45.87±7.62)%。结论Ber具有抑制结肠癌细胞HT-29增殖并诱导其凋亡的作用,此作用可能与Ber抑制肿瘤细胞细胞膜钾离子通道开放有关。
Objective To investigate the effect of berberine on the proliferation and apoptosis of colonic carcinoma cell line HT-29 and clarify its ion mechanism. Methods After treatment with Berberine (0.1-30.0 μmol/L),cell proliferation was assayed by MTT method, cell apoptosis measured by flow cytometry,and IK(V) detected by using patch clamp technique. Results 0. 1-30.0 μmoL/L berberine could inhibit the proliferation of HT-29 cell line significantly ( P 〈 0.05 ). After treatment with 0.1-30.0 μmol/L berberine for 24 h, the number of apoptosis cells was increased dose-dependently from (2.4 ± 1.1)% in PSS group to (7.6 ±1.8)% ,(9.8 ±2.1)% ,(22.3 ±4.2%) ,(40.6 ±4.5)% in berberine group, respectively. 0.3,3.0, and 30.0 μmol/L berberine could inhibit IK(V) of HT-29 cells significantly ( P 〈 0. 01 ). When depolarizing at + 80 mV, the IK (V) of PSS group and 0. 3,3. 0, 30.0 μmoL/L berberine-treated groups was (488 ± 42 ), ( 376 ± 22 ), (296 ± 25 ), and ( 225± 34) pA respectively. The IK (V) in 0.3-30.0 μmol/L berberine-treated groups was ( 77.16 ±5.41 ) %, ( 61.35 ±6.09 ) %, and (45.87 ±7.62) % respectively compared with PSS group. Conclusion Berberine can not only inhibit the proliferation of colonic carcinoma cell line HT-29, but also induce its apoptosis, which may be due to the inhibitory effect of berberine on the delayed-rectifier potassium channel of colonic carcinoma cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第11期1476-1477,共2页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目(2004ABA177)
武汉市晨光计划资助项目(20055003059-15)
关键词
结肠癌
小檗碱
脱噬作用
离子通道
膜片钳
Colonic carcinoma
Berberine
Apoptosis
Lon channel
Patch clamp technique