肉桂提出物甲基查尔斯多酮对单侧肾切除糖尿病大鼠肾脏损害的保护作用

Protective effect of methylhydroxy chalcone polymer extracted from cassia on the renal damage in unilaterally nephrectomized diabetic rats

目的观察肉桂提出物甲基查尔斯多酮（MHCP）对糖尿病肾损害的作用。方法单侧肾切除并腹腔注射链脲佐菌素（STZ）建立大鼠糖尿病模型，随机分为3组，然后灌胃给药12周，测定血管内皮细胞生成因子（VEGF）、Ⅳ胶原（CollogenⅣ）、胰岛素受体底物-1（IRS-1）等指标，并观察大鼠肾脏的组织形态学变化。结果MHCP能够部分降低VEGF（平均相对吸光度为25．3％）、IRS-1（平均相对吸光度为189．5％）的表达，增强大鼠肾脏皮质抗氧化的物质的活性。结论MHCP能够通过提高IRS-1活性增加机体对胰岛素的敏感性、减轻胰岛素抵抗，控制血糖、减少氧化应激和改善内皮细胞功能来减轻糖尿病肾损害。

Objective To elucidate the protective effects of methylhydroxy chalcone polymer （MHCP） extracted from cassia on diabetic kidney damage. Methods Sprague Dawley （SD） rats were peritoneally injected with streptozotocin two weeks after unilateral nephreetomy to establish diabetic animal model, and randomly divided into three groups. They were treated with drugs for 12 weeks. Finally, the expression of VEGF,eollagen Ⅳ and IRS-1 as well as the changes in kidney tissue were investigated. Results Treatment with MHCP clown-regulated partially the expression of VEGF （ relative absorbance was 25.3% ） , IRS-1 （relative absorbance was 189.5% ） , elevated the activities of antioxidants such as SOD, GSH-PX in the cortex of kidney （P 〈 0.05, compared with unilaterally nephrectomized diabetic group）. Conclusion MHCP can ameliorate renal damage in diabetic rats through controlling blood glucose, increasing the activation of IRS-1, reducing oxidative stress, and improving the endothelial function in diabetic rats.