HPLC法测定防风通圣丸中大黄素、大黄酚的含量

Determination of Emodin and Chrysophanol in Fangfengtongsheng Pill by HPLC

目的:建立防风通圣丸中大黄素、大黄酚的含量测定方法。方法:采用高效液相色谱法,色谱柱:Diamonsi C_(18)(250mm×4.6mm,5μm),流动相:甲醇-0.1%磷酸溶液(85:15),检测波长:254nm。结果:大黄素和大黄酚分别在3.95～63.20μg(r=0.999 9)和6.28～100.40μg(r=0.999 3)之间线性关系良好,大黄素的平均加样回收率为98.80%,RSD为0.44%;大黄酚的平均加样回收率为98.88%,RSD为0.13%。结论:该方法准确可靠,分离度好,可用于防风通圣丸的质量控制。

Objective： To establish a method to determine the content of emodin and chrysophanol in Fangfengtongsheng pill. Method： HPLC was used. The separation was performed on Diamonsil C18（250mm× 4.6mm,5 um）with methanol -0.1% phosphoric acid（85： 15） as mobil phase the detecting wavelength was at 254nm. Result： Emodin and chrysophanol showed a good linear relationship within the range of 3.95 - 63.2ug（ r = 0. 999 9） and 6.28 - 100.40ug（ r = 0. 999 3 ）, respectively, the average recovery of emodin was 98.80% with RSD of 0.44%. the average recovery of chrysophanol was 98.88% with RSD of 0.13%, respectively. Conclusion： This method is accurate, and also has good separating degree. It can be used in quality control of Fangfengtongsheng pill.