食管鳞癌中CDC2、MCM2的表达及其临床意义

Expressions of MCM2 and CDC2 in esophageal squamous cell carcinoma and their clinical significance

目的:检测细胞分裂周期2(CDC2)、微小染色体维持蛋白2(MCM2)在食管鳞癌组织中的表达情况,探讨其与食管鳞癌临床病理参数的关系以及在食管鳞癌发生、发展中的作用。方法:应用免疫组化Elivision法检测90例食管鳞癌患者癌组织、28例食管鳞癌患者癌旁正常上皮组织及16例重度不典型增生患者上皮组织中CDC2、MCM2的表达情况。结果:CDC2、MCM2在食管鳞癌组织中的表达明显高于食管癌旁正常上皮、重度不典型增生组织(P<0.01);食管鳞癌组织中CDC2的表达程度与其临床分期有关(P<0.05),MCM2的表达与其分化程度和淋巴结转移均有一定关系(P<0.05);食管鳞癌组织中CDC2和MCM2表达呈正相关关系(P<0.05)。结论:CDC2、MCM2在细胞周期调控中起决定性作用,两者相互协调共同促进了食管鳞癌的发生、发展,可作为临床早期诊断的重要指标。

Objective：To study the expression of CDC2 and MCM2 in human esophageal squamous cell carcinoma（ESCC）,and to investigate their relationship with clinicopathological parameters and their role in the genesis and development of ESCC.Methods：The expressions of CDC2 and MCM2 in 90 cases of human esophageal squamous cell carcinoma,28 cases of adjacent normal tissues and 16 cases of severe dysplasia were determined using Elivision immunohistochemical technique.Results：The positive rates of expressions of CDC2 and MCM2 in ESCC were higher than that in the normal tissue（P〈0.01）;the expression of CDC2 was associated with the clinical staging in ESCC（P〈0.05）.The expression of MCM2 was associated with the differentiation grade and metastasis of lymph nodes in ESCC（P〈0.05）.And there was negatively correlation between CDC2 and MCM2.Conclusions： CDC2 and MCM2 play a crucial role in cell cycle regulation.They together promote the genesis and development of ESCC,and may serve as an important index in early diagnosis of ESCC.