p53及p16基因在甲状腺乳头状癌中的表达及意义

Expressions of p53 and p16 and their clinical significance in thyroid papillary carcinoma

目的:探讨p53及p16基因在甲状腺乳头状癌(papilliary thyroid carcinoma,PTC)中的表达及临床意义。方法:采用免疫组化方法检测157例PTC和30例甲状腺瘤组织中p53及p16基因的表达,并进行统计学分析。结果:PTC中p53的阳性表达率明显高于良性肿瘤(P<0.005),并随着PTC病理分级增高而显著增加(P<0.01),而且颈部淋巴结有转移者高于无转移者(P<0.05);而PTC中p16的阳性表达率明显低于良性肿瘤(P<0.005),且阳性表达率随着PTC病理分级增高而显著降低(P<0.01),p16在发生颈部淋巴结转移的乳头状癌中阳性表达率与未发生转移的乳头状癌中阳性表达率差异有统计学意义(P<0.01)。结论:p53基因的突变及p16基因的失表达都可能促进PTC的发生、发展及其转移,且p16基因可能在PTC的中晚期发挥重要作用,可作为判断PTC恶性程度的参考指标,具有重要临床参考价值。

Objective： To investigate the expressions of p53 and p16 in the papillary thyroid carcinoma （PTC）and their clinical significance. Methods：Immunohistochemistry was used to detect the expressions of p53 and pl6 in 157 cases of PTC and 30 cases of thyroid tumor. Results：The expression of p53 in PTC was significantly higher than that in benign tumors （P 〈 0. 005 ）, and with the increase of the pathological grade of PTC, the expression of p53 increased significantly（ P 〈 0.01 ）, the expression of p53 in cases with neck Lymph node metastasis was higher than that in cases without （ P 〈 0.05 ）, the expression of pl 6 in PTC was significantly lower than that in benign tumors （P 〈 0. 005）, and with the increase of the pathological grade of PTC, the expression of p16 was reduced significantly（ P 〈 0.01 ）, the expression of p16 in cases with neck lymph node metastasis was significantly different compared with that of cases without（P 〈 0.01 ）. Conclusions： The mutation of gene p53 and the loss expression of gene p16 may promote the genesis, progress and metastasis of PTC; gene p16 may play an important role in the middle and advanced stages of PTC,which is of great clinical value and can be used as a reference index in judging the malignant degree of PTC.