栽培大豆和野生大豆亲本及其杂交后代幼苗Cl^-通道基因表达及其与耐氯性的关系

Gene expression of Cl^- channel and its relation to chloride tolerance of Glycine max,Glycine soja and their hybrid seedlings

根据EST数据库拼接了1条大豆Cl-通道基因的EST序列,并通过RT-PCR技术从大豆cDNA中获得该EST。Southern blot、半定量Northern blot、RT-PCR和分光光度法等检测结果表明:该EST所在Cl-通道基因在供试栽培大豆和野生大豆杂交组合(Jackson×BB52)亲本及其耐盐杂交后代JB185(F5)株系等材料中的拷贝数无差异,但在盐胁迫下,栽培大豆Jackson幼苗根和叶中该基因的转录水平表现减弱趋势,而野生大豆BB52和杂交后代JB185(F5)表现增强趋势或相对较高水平,在JB185(F5)中更为明显。盐胁迫下Jackson幼苗体内积累的Cl-相对较多,且向叶片运输比例较大,而BB52和JB185(F5)体内积累的Cl-相对较少,且主要积累在根部,向叶片运输比例较小。

One EST （expressed sequence tag） of chloride channel genes （CLCs） in soybean was assembled according to the EST database, and was confirmed in soybean cDNA by RT-PCR technique. No difference in gene copy number of the EST among the Glycine max （Jackson）, Glycine soja （BB52） and their hybrid F5 （strain JB185） was showed by Southern blot, and its weakening transcriptional level by semi-quantitative Northern blot and RT-PCR exhibited in roots and leaves of Jackson seedlings under salt stress, while an enhanced or relatively higher trend was found in salt-stressed BB52 and JB185 seedlings, especially in JB185. Measured by photometric method, the higher Cl^- content in plants and its larger proportion in leaves of Jackson seedlings, and lower Cl^- content in plants and its larger proportion in roots in BB52 and JB185 seedlings were observed.