水稻紫色叶鞘基因的遗传分析

Genetic Analysis of Gene for Purple Leaf Sheath in Rice

紫色叶鞘是水稻重要的农艺性状。本研究利用带有紫色叶鞘的水稻染色体单片段代换系W23-07-6-02-14与受体亲本华粳籼74杂交,发展F2次级分离群体,对水稻紫叶鞘基因进行鉴定。该代换系的代换区间为RM190-RM204-RM225-RM217-RM253-RM50-RM402-RM539-RM136-PSM388-RM3-RM541-RM162-RM275-RM340,在第6染色体上,叶色基因暂时被命名为PSH(t)。研究发现F1代单株表现为紫色,F2代单株紫色与绿色分离比例符合3∶1,证明紫色是由一对显性基因控制的。本研究为叶色基因的精细定位和克隆奠定了基础。

Purple leaf sheath （laSH） is one of the most important traits in rice genetic improvement. In this study, the single segment substitution line （SSSL） W23 -07 -06 -02- 14 with a substituted interval of RM190 -RM204 - RM225 - RM217 - RM253 - RM50 - RM402 - RM539 - RMI36 - PSM388 - RM3 - RM541 - RM162 - RM275 - RM340 in the 6th chromosome was found to have the gene for purple leaf sheath. It was temporarily designated as PSH（t）. To identify the gene for PSH, an F2 segregating population from the cross between SSSL W23 -07 -06 -02 - 14 and Huajingxian74 was developed. The F1 plants showed PSH trait, and the segregating ratio of purple and green plants in the F2 population was in accordance with 3： 1 ,indicating purple trait was controlled by a couple of major dominant genes. The identification of PSH（t） would be the basis for molecular cloning of the PSH（t） gene.