摘要
硝酸还原酶(NR)是高等植物氮素同化的关键酶,与植物的生长发育有着密切的关系,已被用于作物栽培、育种的早期测定指标之一。然而,在林木上开展的工作不多。由于常规NR活性(即NRA)测定法缺乏对NRA保护因素的深入研究,难以适应次生物质含量高的林木之中的NRA测定,故我们以杨树为材料,对林木NRA的体外测定法进行了较为深入的研究。材料与方法 (一)材料将杨树(Populus deltoides)茎切段置于恒温室中(RH>85％),促进发根后,以1/5Hoagland营养液培养于人工气候室中(30,000lux,25±2℃,RH:75％),新枝长出后。
In nitrate reductase extracting solution, we added excess amount of polyclar AT, and decreased the pH to 7.0, correspondently increased the pH's of the other substrates, thus made the final pH of the reaction system in the range of 7.6—7.8. It was shown that with the treatment of Zn(AC)_2 and PMS to get rid of the excess NADH, factors interfering in nitrite coloration, could be eliminated from this reaction. The inductive conditions of NR and some enzymological characteristics of it have also been studied. Thus a stable and feasible method for the measurement of nitrate reductase activity in vitro in populus leaves has been established.
出处
《林业科学》
EI
CAS
CSCD
北大核心
1990年第3期277-280,共4页
Scientia Silvae Sinicae
关键词
杨树
不溶性PVP
NRA
Populus deltoides
Nitrate inductase
Unsolvable polyclar AT
