彩色豆马勃菌剂的应用研究

A STUDY OF APPLICATION OF DIFFERENT INOCULA OF PISOLITHUS TINCTORIUS TO SOME CHINESE TREE SPECIES

外生菌根真菌——彩色豆马勃(豆包菌)Pisolithus tinctorius(Pers.)Coker and Couch(简称P_t)。应用彩色豆马勃商品化菌剂对我国一些重要用材树种进行菌根化的研究,评价这些树种对P_t菌剂的反应,各种菌剂的效果及其应用前景,为在我国林业生产和环境保护中推广和应用P_t技术及其菌剂提供基础。

This paper is studied the inoculation for development of ectomycorrhizae on container-grown tree seedlings of some commercially important Chinese tree species with two different types of inocula of the ectomycorrhizal fungus Pisolithus tinotorius (P_t). Results show that the inoculation of these tree species tested is successful with solid-base cultures of mycelium of this fungus. Excellent P, ectomycorrhizal root de velopment (P^t index 70^+ and P_t ectomycorrhizal percentage 95%) is produced on the seedlings of the four pines tested. The typical (large, swollen, golden, bifurcate) P_t ectomycorrhizae observed on these four Chinese species are identical to those observed on Loblolly Pine. It confirms that like Loblolly Pine these pine species are hosts of P_t fungus and implys that there is a great potential to use the techniques for development of ectomycorrhizae on these species in forest production. However, spore pellet inoculum is considerably less effective (P_t index 10 and P_t ectomycorrhizal percentage<20%). No ectomycorrhizae on Chinese Fir, Chinese Arborvitae and Siberian Eim are observed in this experiment. This suggests that these species most likely are endomycorrhizal or/and facultative ectomycorrhizal tree spectes. Besides, this study also provides techniques and methods for development and evaluation of ectomycorrhizae on container-grown tree seedlings for forest research and production in China.