摘要
目的:研究青心酮(DHAP)联合St.ThomasⅡ停跳液对离体大鼠心脏缺血再灌注后心肌功能的影响。方法:以离体大鼠工作心脏模型,对比青心酮联合St.ThomasⅡ停跳液与单纯St.ThomasⅡ停跳液,心肌缺血再灌前后左室压(LVP)、左室舒张末期压(LVDEP)、左心室内压上升及下降最大速率(±dp/dt max)、主动脉压(AP)、冠脉流量(CF)、主动脉流量(AF)、每搏心输出量(SV)和测定冠脉流出液乳酸脱氢酶(LDH),心肌三磷酸腺苷(ATP)含量、超氧化物歧化酶(SOD)活性、脂质过氧化物(LPO)含量。结果:青心酮联合St.ThomasⅡ停跳液产生较好的心肌保护作用,明显促进心肌缺血再灌后心肌功能的恢复,增进心脏的收缩功能、心肌ATP含量和SOD活性的恢复,减少LDH的漏出。结论:青心酮联合St.ThomasⅡ停跳液对心脏模拟体外循环的缺血再灌损伤具有保护作用,该方法有临床应用价值。
Objective To investigate the protective effect of 3,4-dihydroxyacetophenone（DHAP） in St.Thomas Cardioplegia Solution in isolated rat hearts which suffer from ischemia/reperfusion injury. Methods The model of Langendorff perfused isolated rat heart was used to study the effects of DHAP on left ventricular pressure（LVP）, left ventricular end-diastolic pressure（LVDEP）, the maximal rate of rise and fall in left ventricular pressure （±dp/dt max）, aortic pressure（AP）, coronary flow（CF）, aortic flow（AF）, activities of lactate dehydrogenase （LDH）, superoxide dismutase（SOD）, and lipid peroxidation（LPO）,concentrations of ATP level pre-and post- ischemia/ reperfusion. Results Compared with only St.Thomas group, DHAP＋ St.Thomas group enhanced the recovery of cardiac hemodynamics and promoted the recovery of contractile function, increased ATP level and SOD activity, and decreased leakage of intracellular lactic dehydrogenase. Conolusion DHAP have effects of anti-ischemia and reperfusion injury, and may play an important role in the future clinic cardiac surgery.
出处
《深圳中西医结合杂志》
2009年第5期279-282,共4页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
