离体培养条件下核桃器官发生和体细胞胚胎发生

ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM THE CULTURE OF WALNUT (JUGLANS REGIA L.)

采自幼树、成年树及成年树伐根萌条的嫩梢腋芽,在改良DKW培养基+6-BA1.0mg/L中可伸长生长;在加有6-BA1.0mg/L+IBA 0.01mg/L的继代培养基中,可保持腋芽的生长并形成不定芽。芽增殖率为每月600％左右。芽苗经5.0mg/L IBA处理7天,然后在含活性炭的无激素培养基中培养20天,有54％可生根成苗。5月中旬至6月上旬的幼胚,在改良DKW培养基+6-BA1.0 mg/L中,黑暗培养30天左右可产生体细胞胚,并可连续多代保持胚性分生能力。体胚在无激素的发芽培养基中黑暗培养7天左右可发芽成苗。胚性愈伤组织在悬浮振荡培养中可保持分生能力。

Axillary shoots were induced with modified DKW medium + 6-BA 1.0mg/L from the dormant axillary buds collected from young trees,mature trees and stump sprouts of walnut. The formation of adventitious multiple shoots was observed in the subculture with modified DKW medium + 6-BA 1.0mg/L+IBA 0.01mg/L. The efficiency of shoots multiplication was near 600% in one month. After being treated in the medium with IBA 5.0 mg/L for 7 days then cultured the medium contained activated carbon, but without growth regulators for 20 days, 54% shoots rooted. Somatic embryos were induced in darkness from immature embryos (from the middle of May to the early of June) cultured in the modified DKW medium+6-BA 1.0mg/L for 30 days. The somatic embryos germinated in the germinating medium without growth regulators within 7 days in darkness. Suspension culture was established from the embryogenetic callus of walnut.