移植瘤体积不同计测方法的比较

Comparative Study of the Different Calculating and Measuring Methods in the Implanted Tumor Volume

目的比较不同计算和测量方法评价移植瘤体积的异同,以探索最佳的接近真实体积的计算和测量方法。方法利用裸鼠皮下移植人原发性肝癌细胞株SMMC-7721建立人肝癌移植瘤动物模型。将荷瘤裸鼠随机分为三组,分别注射PBS液、Ad-LacZ、Ad-p27mt。处理后2周、3周分别处死裸鼠,在体体外内测量移植瘤的长径(a),宽径(b)及高(c),通过4种不同的体积测量公式(πabb/6、abb/2、aab/2、πabc/6)计算出移植瘤体积。同时测出移植瘤近似真实体积。结果πabc/6法计算体积与近似真实体积无明显差异性(P>0.05),πabb/6、abb/2、aab/2法与近似真实体积有明显差异性(P<0.05)。πabc/6法计算体积(cm3)、近似真实体积(cm3)与其重量(g)无明显差异性(P>0.05),而πabb/6、abb/2、aab/2法计算体积(cm3)与重量有明显差异性(P<0.05)。在体体内外方法测得的数据通过四种方法计算出来的体积均无明显差异(P>0.05)。结论4种不同的体积测量公式(πabb/6、abb/2、aab/2、πabc/6),以V=πabc/6方法计测移植瘤体积最准确、客观。在体移植瘤亦可通过游标卡尺测量肿瘤高度,通过v=πabc/6法准确计算出移植瘤体积及推算出移植瘤重量。

Objective To explore the optimum method to evaluate the implanted tumor volume through comparing the different calculating and measuring system. Methods The implanted tumor model was established via the subcutaneous injection method of human primary liver cancer cell line SMMC-7721 into the BAIB/C nude mice. The miees were randomly divided into three groups ： PBS group, Ad-LacZ, Ad-p27mt. Two and three weeks after treatment, in vitro and in vivo, tumor length （a）, wide （b） and high （c） of the implanted tumors were measured, and its volumes were analyzed through four different formulas：πabb/6, abb/2, aab/2, πabc/6. Simultaneously, the real volumes of the implanted tumors were also measured. Results The volumes of the implanted tumors were no significant difference via πabc/6 methods compared to the real weight and volume, but πabb/6, abb/2, and aab/2. Furthermore, there was no significant difference in the volumes of the implanted tumors among the methods of πabb/6, abb/2, aab/2, and πabc /6 in vitro and in vivo. Conclusion The results through πabc/6 method could be optimal among 4 different methods （πabb/6, abb/2, aab/2, πabe/6） . And the volume and weight of the implanted tumors could be evaluated through the πabc/6 method after the high of the implanted tumors were measured by a vernier caliper.