不同nPCL/HA电纺纤维薄膜对人骨髓间充质干细胞黏附率的影响

Effect of various electrospun nPCL/HA scaffold materials on the adhesion rate of hBMSCs

目的:观察nPCL/HA电纺纤维取向薄膜材料对人骨髓间充质干细胞(hBMSCs)的黏附生长的影响,评价其作为组织工程支架材料的应用前景。方法:将体外诱导培养的人骨髓间充质干细胞(hBMSCs),经传代培养第5代的人骨髓间充质干细胞,以2×105的密度与不同nPCL/HA电纺纤维取向薄膜支架在培养板内共培养,同时以nPCL电纺纤维非取向薄膜材料作为对照,初步观察不同nPCL/HA支架材料与hBMSCs的复合培养情况,计算各材料的细胞黏附率。结果:hBMSCs与4种电纺薄膜支架材料均有良好的细胞相容性,细胞能在不同材料表面黏附生长。但是nPCL/80℃HA电纺纤维取向薄膜材料黏附率(45.6±3.2)%,明显高于nPCL/180℃HA电纺纤维取向薄膜材料黏附率(36.5±3.0)%。结论:80℃条件下合成的nPCL/HA电纺取向薄膜材料细胞黏附率较高,较适合作为支架材料应用于hBMSCs为种子细胞的组织工程构建。

Objective To investigate the effect of different nPCL/HA scaffold materials on the adhesive growth of hBMSCs.Methods Electrostatic spinning or electrospinning is an interesting method for producing nonwoven fibers with diameters of submicrometers down to nanometers.Nanofibrous membranes were used in many biomedical applications including drug delivery,wound healing and scaffolding for tissue engineering.Novel bone-scaffolding materials were successfully fabricated by electrospinning from polycaprolactone（PCL） solutions containing nanoparticles of hydroxyapatite（HA）.In intro cultured hBMSCs（5th generation） were seeded at the density of 2 ×105 cell/cm2 onto scaffolds of nPCL/HA and nPCL as control.The cell-material complex was observed in order to evaluate the interaction between cells and materials.The cell adhesion rate of each material was calculated by the method of indirect cell count.Results HBMSCs were shown good adhesion to all 4 types of scaffolds after seeding.The cell adhesion rate of nPCL/ HA（80℃）（45.6±3.2）% was significantly higher than that of nPCL/HA（180℃）（36.5±3.0）%（P〈0.05）.Conclusion Nano-PCL/HA（80℃） was shown significantly higher adhesion rate to hBMSCs,and could be used as scaffold material in the field of bone tissue engineering.