期刊文献+

肝动脉灌注化疗后肝细胞癌8-OHdG、p53和p21^waf1/cip1的表达研究 被引量:1

Oxidative stress in fiver tissues in HCC patients after TACE
原文传递
导出
摘要 目的探讨肝细胞癌(hepatoceilular carcinoma,HCC)患者经导管肝动脉灌注化疗(transcatheter arterial chemotherapy,TAC)后肝组织8-羟基-2′脱氧鸟嘌呤核苷(8-hydroxy-2′-deoxyguanosine,8-OHdG)、p53和p21^waf1/cip1蛋白的表达。方法HCC患者分为两组,术前行TAC治疗的HCC患者39例(TAC组),术前未行TAC治疗的HCC患者50例(非化疗组),肝血管瘤和肝内胆管结石患者15例为对照组。免疫组织化学法检测肝组织的8-OHdG、p53和p21^waf1/cip1蛋白表达水平。结果8-OHdG表达在癌组织中表现为非化疗组高于TAC组(F=9.516,P〈0.05),对照组最低(F=9.516,P〈0.01);在TAC组与非化疗组均为癌组织高于癌旁组织(分别t=7.101,t=8.020,均P〈0.001);癌旁组织与对照组的8-OHdG水平差异无统计学意义;TAC组与非化疗组中的癌组织和癌旁组织的8-OHdG表达呈正相关(r=0.651,r=0.493,均P〈0.001)。p53表达在TAC组和非化疗组差异无统计学意义。p21^waf1/cip1在三组间有差异表达。在癌组织中和癌旁组织p21^waf1/cip1表达均为对照组最高(F=13.459,F=16.613,均P〈0.001),TAC组高于非化疗组(F=13.459,F=16.613,均P〈0.01),但癌组织和癌旁组织比较差异无统计学意义。21^waf1/cip1在非化疗组中癌组织和癌旁组织表达的水平呈正相关(r=0.872,P〈0.001)。结论HCC癌组织的8-OHdG、p53和p21^waf1/cip1的表达高于癌旁和非HCC肝组织;癌细胞可能经由增强的氧化应激修复机制逃脱介入化疗。 Objective To investigate the levels of oxidative stress in liver tissues of hepatocelluar carcinoma ( HCC ) patients after transcatheter arterial chemotherapy ( TAC ). Methods Immunohistochemistry streptavidin biotinylated peroxidase (S-P) method was used to detect the cellular levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) , p53 and p21^waf1/cip1. Eighty-nine HCC patients were divided into TAC group (39 cases) and Non-TAC group (50 cases). 15 Non-HCC liver tissues served as controls. Result 8-OHdG level was higher in Non-TAC group than that in TAC group in tumor tissues (F=9. 516, P 〈 0. 05), with that being the lowest in control group (F = 9. 516, P 〈 0. 01 ) ; 8-OHdG levels in cancer tissues were significantly higher than that in tumor surrounding tissues in both TAC group (, t = 7. 101, P 〈 0. 001 ) and Non-TAC group ( t = 8. 020, P 〈 0.001 ) , there was no significant difference of 8-OHdG levels between para-tumor tissues and controls. The levels of 8-OHdG between tumor and its surrounding tissues in TAC group (r = 0. 651 , P 〈 0. 001 ) and non-TAC group (r = 0. 493, P 〈 0. 01 ) was in positive correlation. The difference of p53 levels in cancer tissues in TAC group and Non-TAC group were not statistically significant and p53 was not detected in para-tumor tissues. The difference of p21^waf1/cip1 levels among TAC group, Non-TAC group and controls was statistically significant, the levels of p2^waf1/cip1 in normal group was the highest ( F = 13.459, P 〈 0. 001 ), followed by that in TAC and Non-TAC group in cancer tissues ( TAC vs. Non-TAC group, P 〈 0. 01 ) ; p21^waf1/cip1 expression in normal controls was significantly higher than that in both TAC and Non-TAC group in para-tumor tissues ( F = 16. 613, P 〈 0. 001 ). The correlation of p21^waf1/cip1 levels between tumor and its surrounding tissues was significant in non- TAC group ( r = 0. 872, P 〈 0. 001 ). Conclusions Oxidative stress levels in HCC tumor tissues were higher than in para-tumor tissues and non-HCC liver tissues. Cancer ceils probably survive chemotherapy by fortifying oxidative stress repair mechanism.
出处 《中华普通外科杂志》 CSCD 北大核心 2009年第10期795-798,共4页 Chinese Journal of General Surgery
基金 国家自然科学基金资助项目(30460143,30560133)
关键词 肝细胞 基因表达 化学疗法 肿瘤 局部灌注 Carcinoma, hepatocellular Gene expression Chemotherapy, cancer, regional perfusion
  • 相关文献

参考文献12

  • 1Faber M,Coudray C,Hida H,et al.Lipid peroxidation products,and vitamin and trace element status in patients with cancer before and after chemotherapy,including adriamycin.A preliminary study.Biol Trace Elem Res,1995,47:117-123.
  • 2郭雅,彭涛,刘志明,刘唐威,李佳荃,黎乐群.术前化疗对DNA修复酶hOGG1在肝癌组织中表达的影响[J].中华普通外科杂志,2005,20(8):516-518. 被引量:1
  • 3Kasai H.Analysis of a form of oxidative DNA damage,8-hydroxy-2'-deoxyguanosine,as a marker of cellular oxidative stress during carcinogenesis.Mutal Res,1997,387:147-163.
  • 4Erhola M,Toyokuni S,Okada K,et al.Biomarker evidence of DNA oxidation in lung cancer patients:association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy,chemotherapy,and response to treatment.FEBS Lett,1997,409:287-291.
  • 5Toyokuni S,Okamoto K,Yodoi J,et al.Persistent oxidative stress in cancer.FEBS Lett,1995,358:1-3.
  • 6Conklin KA.Chemotherapy-associated oxidative stress;impact on chemotherapeutic effectiveness.Integr Cancer Ther,2004,3:294-300.
  • 7Matsumoto K,Satoh Y,Sugo H,et al.Immunohistochemical study of the relationship between 8-hydroxy-2'-deoxyguanosine levels in noncancerous region and postoperative recurrence of hepatocellular carcinoma in remnant liver.Hepatol Res,2003,25:435-441.
  • 8Levine AJ,Momand J,Finlay CA.The p53 tumour suppressor gene.Nature,1991,351:453-456.
  • 9张晓晖,王文亮.肝细胞肝癌组织中p21^(WAF1)的表达及其意义[J].中华医学杂志,1998,78(1):74-75. 被引量:9
  • 10Hui AM,Kanai Y,Sakamoto M,et al.Reduced p21^waf1/cip1 expression and p53 mutation in hepatocellular carcinomas.Hepatology,1997,25:575-579.

二级参考文献3

共引文献8

同被引文献5

  • 1Liu Zhiming,Li Lequn,Peng Minhao,et al. Hepatitis B virus infection eontributes to oxidative stress in a population exposed to aflatoxin B1 and high risk for hepatocellular carcinoma[J]. Cancer Lett, 2008,263 (2) : 212- 222.
  • 2Nishikawa T, Nakajima T, Katagishi T, et al. Oxida- tive stress may enhance the malignant potential of human hepatocellular carcinoma by telomerase activation [J].Liver International,2009,29(6) : 846-856.
  • 3Maier J, van Steeg H, van Oostrom C, et al. Deoxyribonucleic Acid Damage and Spontaneous Mutagenesis in the Thyroid Gland of Rats and Miee[J]. Endocrinology, 2006,147(7) :3 391-3 397.
  • 4郭玉荣.免疫组化染色的影响因素与质量控制[J].国际检验医学杂志,2009,30(11):1120-1120. 被引量:3
  • 5邓娟,周华东,陈曼娥,张莉莉.局灶性脑缺血-再灌注大鼠神经元DNA氧化损伤的研究[J].中国危重病急救医学,2001,13(8):478-480. 被引量:12

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部