摘要
目的探讨肝细胞癌(hepatoceilular carcinoma,HCC)患者经导管肝动脉灌注化疗(transcatheter arterial chemotherapy,TAC)后肝组织8-羟基-2′脱氧鸟嘌呤核苷(8-hydroxy-2′-deoxyguanosine,8-OHdG)、p53和p21^waf1/cip1蛋白的表达。方法HCC患者分为两组,术前行TAC治疗的HCC患者39例(TAC组),术前未行TAC治疗的HCC患者50例(非化疗组),肝血管瘤和肝内胆管结石患者15例为对照组。免疫组织化学法检测肝组织的8-OHdG、p53和p21^waf1/cip1蛋白表达水平。结果8-OHdG表达在癌组织中表现为非化疗组高于TAC组(F=9.516,P〈0.05),对照组最低(F=9.516,P〈0.01);在TAC组与非化疗组均为癌组织高于癌旁组织(分别t=7.101,t=8.020,均P〈0.001);癌旁组织与对照组的8-OHdG水平差异无统计学意义;TAC组与非化疗组中的癌组织和癌旁组织的8-OHdG表达呈正相关(r=0.651,r=0.493,均P〈0.001)。p53表达在TAC组和非化疗组差异无统计学意义。p21^waf1/cip1在三组间有差异表达。在癌组织中和癌旁组织p21^waf1/cip1表达均为对照组最高(F=13.459,F=16.613,均P〈0.001),TAC组高于非化疗组(F=13.459,F=16.613,均P〈0.01),但癌组织和癌旁组织比较差异无统计学意义。21^waf1/cip1在非化疗组中癌组织和癌旁组织表达的水平呈正相关(r=0.872,P〈0.001)。结论HCC癌组织的8-OHdG、p53和p21^waf1/cip1的表达高于癌旁和非HCC肝组织;癌细胞可能经由增强的氧化应激修复机制逃脱介入化疗。
Objective To investigate the levels of oxidative stress in liver tissues of hepatocelluar carcinoma ( HCC ) patients after transcatheter arterial chemotherapy ( TAC ). Methods Immunohistochemistry streptavidin biotinylated peroxidase (S-P) method was used to detect the cellular levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) , p53 and p21^waf1/cip1. Eighty-nine HCC patients were divided into TAC group (39 cases) and Non-TAC group (50 cases). 15 Non-HCC liver tissues served as controls. Result 8-OHdG level was higher in Non-TAC group than that in TAC group in tumor tissues (F=9. 516, P 〈 0. 05), with that being the lowest in control group (F = 9. 516, P 〈 0. 01 ) ; 8-OHdG levels in cancer tissues were significantly higher than that in tumor surrounding tissues in both TAC group (, t = 7. 101, P 〈 0. 001 ) and Non-TAC group ( t = 8. 020, P 〈 0.001 ) , there was no significant difference of 8-OHdG levels between para-tumor tissues and controls. The levels of 8-OHdG between tumor and its surrounding tissues in TAC group (r = 0. 651 , P 〈 0. 001 ) and non-TAC group (r = 0. 493, P 〈 0. 01 ) was in positive correlation. The difference of p53 levels in cancer tissues in TAC group and Non-TAC group were not statistically significant and p53 was not detected in para-tumor tissues. The difference of p21^waf1/cip1 levels among TAC group, Non-TAC group and controls was statistically significant, the levels of p2^waf1/cip1 in normal group was the highest ( F = 13.459, P 〈 0. 001 ), followed by that in TAC and Non-TAC group in cancer tissues ( TAC vs. Non-TAC group, P 〈 0. 01 ) ; p21^waf1/cip1 expression in normal controls was significantly higher than that in both TAC and Non-TAC group in para-tumor tissues ( F = 16. 613, P 〈 0. 001 ). The correlation of p21^waf1/cip1 levels between tumor and its surrounding tissues was significant in non- TAC group ( r = 0. 872, P 〈 0. 001 ). Conclusions Oxidative stress levels in HCC tumor tissues were higher than in para-tumor tissues and non-HCC liver tissues. Cancer ceils probably survive chemotherapy by fortifying oxidative stress repair mechanism.
出处
《中华普通外科杂志》
CSCD
北大核心
2009年第10期795-798,共4页
Chinese Journal of General Surgery
基金
国家自然科学基金资助项目(30460143,30560133)
关键词
癌
肝细胞
基因表达
化学疗法
肿瘤
局部灌注
Carcinoma, hepatocellular
Gene expression
Chemotherapy, cancer, regional perfusion