结缔组织生长因子siRNA对大鼠血管平滑肌细胞增殖和迁移的影响

Effect of Connective Tissue Growth Factor siRNA on the Proliferation and Migration of Vascular Smooth Muscle Cell in Rat

目的观察结缔组织生长因子(CTGF)siRNA对CTGF表达及对大鼠血管平滑肌细胞(VSMC)增殖和迁移的影响。方法培养大鼠血管平滑肌细胞,用脂质体法将抗CTGF483siRNA进行转染,Western blot法检测转染效果,MTT法及划痕实验测定对VSMC增殖和迁移的影响。结果转染CTGF483siRNA组的CTGF表达明显低于未转染组,MTT法显示转染CTGF483siRNA后VSMCs增殖减慢,划痕试验证实转染CTGF483siRNA后VSMCs迁移受到抑制。结论CTGFsiRNA可以抑制VSMC增殖和迁移,有望为颈动脉粥样硬化性缺血性脑血管病的预防和治疗新靶点。

Objective To observe the effect of the connective tissue growth factor （CTGF） siRNA on the proliferation and migration of the vascular smooth muscle cells （VSMCs） in rat. Methods Primary VSMCs were cultured and CTGF483 siRNA was transfected into VSMCs, and effect of transfection was detected by Western blot. The proliferation and migration of VSMCs were determined by MTT and scratch wound assay respectively. Results CTGF expression was significantly decreased by Western blot and the proliferation of VSMCs was decreased by MTT method in CTGF483 siRNA transfected VSMCs compared with control. Scratch wound assay showed that the migration of VSMCs was inhibited after transfection. Conclusion Specific down-regulation of CTGF expression could inhibit the proliferation and migration of VSMCs, which may provide a new method for the prevention and treatment of ischemic cerebral vascular disease caused by carotid atherosclerosis.