小鼠纹状体中神经递质相关基因表达的节律性波动

Rhythmic Oscillation of Neurotransmitter-related Genes Expression in the Mouse Striatum

目的探讨纹状体神经肽强啡肽(dynorphin,DYN)、脑啡肽1(enkephalin1,ENK1)、P物质(substance P,SP)、胆囊收缩素(cholecystokinin,CCK)和多巴胺受体1和2(dopamine receptor1and2,D1r和D2r)是否受分子时钟机制的调控,它们的节律变化是否与时钟基因有关。方法分别对刚出生(产后3d)、断奶前(产后14d)和成年(产后60d)小鼠纹状体中上述基因24小时的mRNA用实时定量RT-PCR的检测方法观察其节律变化。结果产后3d小鼠纹状体内没有观察到相关基因的节律性变化;产后14d时,纹状体内D2r、DYN和ENK1基因随时间呈现节律变化,出生后60d时,纹状体内除D1r外,所有基因表达均随时间昼夜波动。结论小鼠纹状体中神经递质相关基因的昼夜表达变化规律是在出生后逐渐形成的。

Objective The aims of this study were to determine whether striatal neuropeptides （dynorphin （DYN）, enkephalin 1 （ENK1）, substance P（SP）, eholecystokinin （CCK）） and dopamine receptor 1 and 2 （Dlr and D2r） are regulated by the molecular clock; whether their rhythmic changes depend on the matural molecular clock. Methods Twenty-four hour mRNA oscillations of these genes were evaluated in the mouse striatum at early postnatal （postnatal day 3 ）, pre-weaning（ postnatal day 14）, and adult（ postnatal day 60） stages. At P3, no daily oscillations were observed. Results A significant time effect was present for D2r, DYN, and ENK1 at P14, and for all genes except Dlr, at P60. Conclusion Circadian expression of these neurotransmitter-related genes develops in the mouse striatum gradually.