α-突触核蛋白促进大鼠原代培养神经元突起生长

α-Synuclein Promotes Neurite Outgrowth of Primarily Cultured Rat Brain Neurons

目的研究人α-突触核蛋白(α-Synuclein,α-Syn)对神经元突起生长的作用。方法原代培养新生大鼠大脑皮质神经元,细胞外添加α-Syn蛋白,测定不同时间神经元突起的长度;观察添加不同浓度的α-Syn蛋白后神经元突起的生长情况。结果α-Syn处理组神经元突起的平均长度在培养1h、2h和4h时均明显大于对照组,α-Syn的这一作用可被其特异性单克隆抗体阻断;向培养基中添加不同浓度的α-Syn后,神经元突起的长度随α-Syn浓度的增加而增加。结论α-Syn具有促进神经元突起生长的作用,并且呈明显的量-效关系。

Objective To investigate the effect of α-Synuclein （ct-Syn） on the neurite outgrowth of brain neurons. Methods Neurons isolated from the neocortex of newborn rats were cultured and α-Syn was added into the culture medium to observe the effect of the protein on the neurite outgrowth of the neurons. In addition, the effect of different concentrations of α-Syn on the neurite outgrowth was studied. Results The mean neurite length of the neurons treated with α-Syn was significantly longer than that of control neurons at 1 h, 2 h and 4 h after adding α-Syn. The effect of α-Syn on neurite outgrowth was enhanced with the increase of α-Syn concentration and was reversed by anti-α-Syn monoclonal antibody. Conclusion α-Syn can promote neurite outgrowth of primary rat brain neurons in a dosedependent manner.