吲哚乙酸联合辣根过氧化物酶对人SACC-2细胞的抑制作用

Combined use of indole-3-acetic acid and horseradish peroxidase inhibits the growth of human adenoid cystic carcinoma SACC-2 cells

目的:探讨吲哚乙酸(IAA)与辣根过氧化物酶(HRP)在体外共同作用对人涎腺腺样囊性癌细胞SACC-2生长抑制的影响.方法:用不同浓度的IAA/HRP(IAA20、40、60、80、100μmol/L+HRP1.2mg/L)与SACC-2细胞共同培养48h后,光镜下观察癌细胞的生长;CCK-8法测定细胞生长抑制率;半定量RT-PCR技术检测caspase-3mRNA水平.结果:与阴性对照组比较,48h后SACC-2细胞皱缩,变小变圆,空泡明显;40、60、80、100μmol/LIAA组的细胞生长抑制率明显增高(31.8%,38.71%,60.57%,80.18%,均P<0.05);半定量RT-PCR结果显示,60、100μmol/LIAA与1.2mg/LHRP联合作用可上调caspase-3mRNA水平(0.835±0.019,1.667±0.022vs0.242±0.025,均P<0.01).结论:IAA/HRP对人涎腺腺样囊性癌细胞SACC-2生长有明显的抑制作用,可通过激活caspase-3mRNA的表达,诱导凋亡.

AIM：To investigate the effects of combined use of indole-3-acetic（IAA）and horseradish peroxidase（HRP）on the growth of human adenoid cystic carcinoma SACC-2 cells in vitro. METHODS：After HRP（1.2 mg/L）and IAA at different concentrations（20,40,60,80 and 100 μmol/L）were co-incubated with SACC-2 cells for 48 h,the changes in cell morphology were observed under light microscopy,the growth inhibition rate was measured by the CCK-8 assay, and the expression level of caspase-3 mRNA was determined by semiquantitative RT-PCR. RESULTS：Compared with negative control cells,cells incubated with IAA/HRP underwent shrinkage,became small and round,and showed significant vacuole formation.The growth inhibition rates achieved in cells incubated with HRP（1.2 mg/L）and IAA at 40,60,80 and 100 μmol/L were significantly higher than that in control cells（31.8%,38.71%,60.57%and 80.18% vs 0%,respectively;all P〈0.05）.Combined use of HRP（1.2 mg/L）and IAA at 60 and 100μmol/L could significantly upregulate the expression of caspase-3 mRNA when compared to control cells（0.835±0.019 and 1.667±0.022 vs 0.242± 0.025,respectively;both P〈0.01）. CONCLUSION：Combined use of IAA and HRP is able to significantly inhibit the growth of SACC-2 cells in vitro perhaps through upregulation of caspase-3 mRNA expression to induce cell apoptosis.