家猫高眼压模型的建立及特征分析

Intraocular hypertension in cat model:building and analysis

目的:探讨家猫高眼压模型的建立方法及特点。方法:健康成年家猫10只20眼,1眼采用单侧眼前房注入20g/L甲基纤维素的方法建立高眼压模型,另1眼作对照,从高眼压模型标准及多焦视网膜电图(mfERG)两方面进行模型评价。结果:基线眼压为15mmHg,10眼在第1次注药后均出现高眼压,其中6眼高眼压持续时间为7～15d,平均高眼压28～39mmHg,眼压峰值35～49mmHg,一次注药模型成功率为60%。在造模后的不同时间,模型眼眼压明显升高,与造模前及对照眼比较均有极显著性差异(P<0.01)。多焦视网膜电图一阶反应在家猫造模后急性眼压升高时有明显的变化。造模后P1波总的反映密度减弱,与术前比较差异有统计学意义(P<0.05);同心环各环P1波反映密度均较术前减弱,差异有统计学意义(P<0.01);第3环P1波峰潜时延长,较术前差异有统计学意义(P<0.05)。结论:前房注入20g/L的甲基纤维能够建立高眼压模型,mfERG改变高眼压模型判定标准。

AIM： To explore modeling method and characteristic of intraocular hypertension in cat. METHODS： Ten healthy adult cats （20 eyes） were involved in this study. Intraocular hypertension was build by injecting 20g/L cellogran into anterior chamber of single eye,with another eye being control group. And the model was assessed according to the hypertensive model standard and multifocal electroretinogram （mfERG）. RESULTS： The basic intraocular pressure （lOP） was 15mmHg. 10 eyes showed hypertension after the fist injection while 6 of those lasted 7-15 days. The average lOP ranged from 28 to 39 mmHg, and peak IOP was 35 to 49 mmHg. The success ratio for this model was 60% after single injection. The IOP of the model eyes obvious-ly increased at different time points after injection, and showed significant difference when compared with eys before modeling and the control eyes. The mfERG changed obviously in the first order kernel after acute lOP increased. Total response density of wave P1 after modeling were lower than those before modeling （P 〈 0. 05）. The response densities of wave P1 of each rings decreased after modeling （ P 〈 0.01 ）. The peak latency of the third ring after modeling was longer than before, with significant difference （ P〈 0.05）. CONCLUSION： Intraocular hypertension in cat models can be established by injecting 20g/L cellogran into anterior chamber and will last for over one week. Changes of the mfERG accord with the standards of alaucoma in animal model.