离心速度及时间对培养细胞透射电镜样品制备的影响

The influence of centrifugal speed and time on TEM sample preparation of culture cell

目的:探讨消化离心法中不同的离心条件对培养细胞超微结构的影响,寻找最佳的离心速度及时间。方法:将培养细胞消化后分成不同的离心速度和时间组,电镜下比较各组细胞超微形态的变化。结果:当离心速度低于800r/min,8min时,无法收集到足够的细胞量。离心速度2000r/min,15min时细胞挤压变形,胞浆出现撕裂等人工假象。离心速度在800～1500r/min,8～15min时制样较容易,细胞超微结构保存良好。结论:消化离心法制备培养细胞透射电镜样品,其最佳的离心速度和时间是800～1500r/min,8～15min。

Objective：To investigate the influences of different centrifugal conditions on the ultrastructure of cultured cells in the digestion centrifugation to find out the best speed and time of centrifugation.Methods：The cultured cells after digested,were divided into groups according to different digestion centrifuge speed and time,and the changes of cell morphology in each group at different centrifugation time and speed were compared under transmission electron microscope.Results：Enough cells could mot be collected with the centrifuge speed less than 800 r/min,8 min.The cells were deformed due to extrusion or the cytoplasm was torn with the speed of 2 000 r/min,15 min.With the speed of 800-1 500 r/min,8-15 min,it was more proper to prepare samples and preserve cell ultrastructure.Conclusion：It is ideal to prepare TEM samples of cultured cells by using the cells being digested and centrifuged with speed at 800 1 500 r/min and time of 815 minutes.