VEGF和BMP-2在骨肉瘤组织中的表达及临床意义

Expression of VEGF and BMP-2 in osteosarcoma tissue and its clinical significance

目的:探索VEGF和BMP-2在骨肉瘤组织中的表达与骨肉瘤的诊断、转移复发及预后的关系。方法:应用免疫组织化学法(S-P法)检测41例骨肉瘤组织中VEGF、BMP-2及CD34的表达,计算微血管密度(MVD),并综合分析所有病例资料。结果:41例骨肉瘤患者中,VEGF及BMP-2阳性表达率分别为68.2%(28/41)和61.0%(25/41),与肿瘤大小、Price’s分级、Dahlin’s组织学分型等无关(P>0.05)。VEGF阳性组BMP-2阳性率及MVD(分别为75%和40.25±8.02)与阴性组BMP-2阳性率及MVD(分别为30.8%和15.54±5.87)相比,差异有显著意义(P<0.05)。复发或转移组VEGF及BMP-2的阳性率(分别为92.9%和85.7%)明显高于无复发或转移组的VEGF及BMP-2的阳性率(分别为55.6%和48.1%),差异具有显著意义(P<0.05)。VEGF及BMP-2阳性组的5年存活率(分别为7.1%和4%)明显低于VEGF及BMP-2阴性组的5年存活率(分别为46.2%和43.8%),差异有显著意义(P<0.05)。结论:VEGF、BMP-2在骨肉瘤组织中呈高表达,VEGF的表达与BMP-2的表达及MVD显著相关,检测VEGF、BMP-2的表达和MVD值可以作为判断骨肉瘤转移复发和预后的重要指标。

Objective：To explore the expression of VEGF and BMP-2 in osteosarcoma tissue and its association with diagnosis,metastasis/recurrence and prognosis of osteosarcoma.Methods：Immunohistochemical staining（SP method）was used to detect the expression of VEGF,BMP-2 and CD34 in specimens from 41 cases of osteosarcoma.Microvessel density was calculated,and overall information of all the 41 cases were analyzed.Results：In 41 cases,the positive rates of VEGF and BMP-2 were 68.2%（28/41）and 61.0%（25/41）respectively.The expression of VEGF and BMP-2 were not significantly correlated to tumor＇s size,Price＇s grade and Dahlin＇s difierentiation（P〉0.05）.The positive rate of BMP-2 and MVD（75% and 40.25±8.02）in VEGF positive patients were respectively much higher than those（30.8%and 15.54±5.87）in VEGF negative patients（P〈0.05）.The positive rates of VEGF and BMP-2（92.9%and 85.7%）in patients with metastasis / recurrence were respectively remarkably higher than those（55.6% and 48.1%）in patients without metastasis/recurrence（P〈0.05）.The five-year survival rate in patients with positive expression of VEGF and BMP-2（7.1%and 4%）were significantly lower than that（46.2%and 43.8%）in negative control group（P〈0.05）.Conclusion：High expression of VEGF and BMP-2 were found in osteosarcoma tissue.The expression of VEGF was closely correlated to the expression of BMP-2 and MVD.Detection of VEGF and BMP-2 expression and MVD may be useful for judging metastasis/recurrence and prognosis of osteosarcoma.