重建肝动脉的大鼠肝移植模型改良的实验研究

Modification of arterialized orthotopic liver transplantation in rats

目的:探讨稳定、高效地建立肝动脉重建的大鼠原位肝移植模型的手术方法。方法:参照"双袖套"法,在供肝的灌注、切取、肝上下腔静脉吻合等方面进行改良,供、受体肝总动脉间用改良的支架法吻合重建肝动脉血供。结果:共完成大鼠原位肝移植80例,供体手术时间:(40±3)min,受体手术时间:(50±5)min,无肝期时间:(16±2)min,肝上下腔静脉吻合时间:(6～9)min,肝动脉吻合时间:(2～3)min。术中无受体死亡,48h存活率为96.3%,1周存活率为93.7%,1月存活率为:87.5%。结论:该方法成活率高,稳定性好,是建立肝动脉重建的大鼠原位肝移植模型的理想方法。

Objective：To improve the surgical technique to establish the arterialized orthotopic liver transplantion in rat.Methods：On the basis of two-cuff technique of Kamada,we improved the techniques of separaring,perfusing donor liver,the anastomosis of the suprahepatic inferior vena cava,and so on.And used the modified ＂stent＂ method to reestablish the hepatic artery.Results：80 cases of operation were completed.The time of donor operation,recipient operation and anhepatic time were（40±3） min,（50±5）min,and（16±2）min,respectively.The time of reestablishing suprahepatic inferior vena cava and hepatic artery were（69） min,（23） min,respectively.The survival rate at 48 h,1 week and 1 month were 96.3%,93.7%,and 87.5%,respectively.Conclusion：The modified method can enhance the stability and survival rate,which is an ideal method for establishing the arterialized orthotopic liver transplantation model in rat.