^(18)F-氟脱氧葡萄糖中微量氨基聚醚(2.2.2)含量测定

Content determination of trace kryptofix 222 in ^(18)F-fluorodeoxyglucose

目的:建立18F-氟脱氧葡萄糖中微量氨基聚醚(2.2.2)紫外分光光度检测方法。方法:在pH为6.4的柠檬酸氢氧化钠缓冲溶液中,加入适量苯甲醛和硝酸铅配制储备溶液,使溶液在250nm检测波长处的紫外吸收曲线为一平台,吸收值在0.4～0.6Abs间。在储备液中加入适量18F-氟脱氧葡萄糖溶液,用分光光度法检测微量氨基聚醚(2.2.2)铅(Ⅱ)络合物吸收值,定量测定氨基聚醚(2.2.2)。结果:在250nm下,氨基聚醚(2.2.2)的线性范围为0.0～10.0μg/ml,络合物摩尔吸光系数为1.6×104,定量限为0.13μg/ml。加样回收率在97.2%～102.7%,平均回收率为99.7%,相对标准偏差2.4%(n=5)。结论:该方法准确、灵敏,可用于18F-氟脱氧葡萄糖中微量氨基聚醚(2.2.2)含量测定。

Objective：To establish an ultraviolet spectrophotometry method for determination of trace kryptofix 2.2.2 in 18F-fluorodeoxyglucose product.Method：A storage solution was prepared by adding properly benzaldehyde and lead nitrate to citric acid-NaOH buffer solution（pH = 6.4）,and ultralviolet absorbance curve of the solution was changed to platform at the wavelength of 250 nm nearby,and the absorbance value of this solution was 0.4Abs 0.6Abs at 250 nm.Properly 18F-fluorodeoxyglucose product was added to storage solution,and the absorbance value of trace Pb（Ⅱ）-kryptofix 222 complex was detected by ultraviolet Spectrometry for quantitative determination of trace kryptofix 222.Results：The absorbance coefficient of complex was 1.6×104 L/（mol·cm） at 250 nm;the linear range of kryptofix 222 was 0.0μg/ml10.0μg/ml,the limit ofkryptofix 222 was 0.0μg/ml10.0μg/ml,the limit of quantification was 0.13μg/ml;the adding recovery was 97.2%～102.7%,with the average recovery of 99.7%;the relative standard deviation（RSD） was 2.4%（n=5）.Conclusion：The method was specific,sensitive and suitable for determination of trace kryptofix 2.2.2 in 18F-fluorodeoxyglucose product.