弓形虫代谢分泌抗原对猪T细胞亚群的影响

Effect of Toxoplasma gondii excreted/secreted antigens on porcine T lymphocyte subgroups

为了研究弓形虫代谢分泌抗原对仔猪T细胞亚群及抗体的影响,将仔猪随机分为6组,每组5只,分别为E/SA组、E/SA+CPG(未乳化)组、E/SA+CPG组、E/SA+IL-2组、E/SA+IL-2+CPG组及对照组。分别于免疫前、免疫后第2周、免疫后第4周、感染后第1周及感染后第2周采集仔猪外周血,用流式细胞仪对各试验组外周血中CD4+及CD8+T淋巴细胞亚群的动态变化规律进行检测,用IHA对抗弓形虫抗体水平做检测。结果显示,弓形虫代谢分泌抗原免疫仔猪后第4周,免疫组外周血CD4+、CD8+T淋巴细胞水平和特异性抗体效价与对照组相比均有大幅度升高;感染后第1周,免疫组CD4+T细胞水平及CD4+/CD8+比值较感染前有不同程度降低,感染后第2周,免疫组CD4+T细胞水平及CD4+/CD8+比值上升至免疫后水平,CD8+水平下降,感染后特异性抗体效价进一步升高。表明,弓形虫代谢分泌抗原能够提高仔猪外周血CD4+及CD8+T淋巴细胞水平和特异性抗体水平。

To elucidate the effects of ToxopIasma gondii excreted/secreted antigen（E/SA） on piglet＇s T lymphocyte subgroups and antibodies, piglets were randomly divided into six groups of E/SA, E/SA＋CpG（unemulsification） ,E/SA ＋ CpG, E/SA ＋ IL-2, E/SA ＋ IL-2 ＋ CpG groups and the control group. The dynamic changes of the CD4^＋ and CD8^＋ T lymphocyte subgroups in the piglet＇ s peripheral blood were determined by flow cytometry, and antibody levels were tested by indirect hemagglutination assay. The results showed that on weeks four post-immunization with T. gondii E/SA, the peripheral blood CD4^＋ and CD8^＋T lymphocyte levels and specific antibody levels of the immune groups had significantly increased compared with those of the control group. The specific antibody levels further increased on week 1 after inoculation,the CD4^＋ T cells levels and CD4^＋/CD8^＋ ratio of the immune groups reduced to varying degrees than two weeks post infection, CD4^＋ T cells levels and CD4^＋/ CD8^＋ ratio in the immune group increased to immunized levels,CD8^＋ levels declined. So the T. gondii E/SA can improve the percentage of the piglet＇s CD4^＋ and CD8^＋ T lymphocytes and the specific antibody levels in peripheral blood.