摘要
目的探讨外周血胰岛素样生长因子Ⅱ(IGF-II)-mRNA在肝癌诊断和判断预后中的价值。方法从癌组织和外周血中制备总RNA,经随机引物和逆转录酶作用合成IGF-ⅡcDNA,再分别以RT-PCR扩增IGF-ⅡcDNA片段,分析其在肝病诊断与鉴别中的临床价值。结果肝癌组织和外周血中扩增出的IGF-Ⅱ基因片段大小为170bp,并与原设计相一致。肝癌、癌灶周围和远癌组织中,IGF-Ⅱ基因检出率分别为100%、53.3%和0。肝癌患者外周血IGF-II基因片段阳性率34.2%,在Ⅰ、Ⅱ和Ⅲ期肝癌中分别为14.3%、19.4%和45.5%,在伴有肝外转移肝癌中全数阳性,在AFP<50ng/ml的17例中阳性6例(35.3%);肝硬化、慢性肝炎、急性肝炎和肝外肿瘤组中未见IGF-Ⅱ基因片段阳性。IGF-Ⅱ基因标志与肝癌大小间差异无统计学意义(P>0.05)。结论外周血IGF-Ⅱ基因分析有助于肝癌的诊断、转移判别及术后复发的监测。
Objective To explore clinical values of peripheral blood insulin-like growth factor Ⅱ （IGF-Ⅱ ）genes markers in diagnosis and differential diagnosis of hepatocellular careinoma（HCC）. Meth- otis Total RNAs were extracted from human hepatoma tissues and peripheral blood and synthesized to IGF-Ⅱ cDNA through random primers and reverse transcriptase,amplified by a nested polymerase chain reaction assay,and the clinical values of IGF-Ⅱ gene marker were analyzed in patients with liver dis- eases. Results The fragments of IGF-Ⅱ mRNA amplified by RT-PCR were identical to the original designed ones with size of 170bp. The fragments of IGF-Ⅱ mRNA were seen with 100% in cancerous, 53.5% in paracancerous and 0 in distal cancerous tissues, respectively. The incidence of peripheral blood IGF-Ⅱ gene fragments was 34.2% in HCC, 0 in liver cirrhosis, chronic hepatitis, acute hepatitis, and extrahepatic tumor, respectively. The presence of IGF- Ⅱ mRNA correlated with the stage of HCC and the persence of intrahepatic metastasis. The IGF- Ⅱ mRNA was found in all metastasis at extrahep- atic organs （100%）, The positive rate of IGF-Ⅱ was 35.5% in HCC patients with AFP〈50ng/ml. No sig- nificant correlation was found between tumer size and IGF-Ⅱ gene expression. Conclusion The pre- sent data suggest that the gene analysis of peripheral blood IGF-Ⅱ is useful for HCC diagnosis ,differ- entiation of extrahepatic metastasis and monitoring postoperative recurrence..
出处
《苏州大学学报（医学版）》
CAS
北大核心
2009年第4期684-687,共4页
Suzhou University Journal of Medical Science
基金
江苏省医学重点人才基金资助项目(RC2003100)
