氮、磷、钾对若干种木本植物离体培养繁殖的影响

EFFECTS OF NITROGEN, PHOSPHORUS AND POTASSIUM ON THE PROPAGATION IN VITRO OF SEVERAL WOODY PLANTS

本文通过培养基中三个主要营养元素氮、磷、钾对若干种木本植物(如杉木、北美红杉等)离体培养繁殖影响的研究,提出了较适合于这些木本植物离体培养的改良MS基本培养基(其中大量元素氮、磷、钾水平修改为:NH_4NO_3 850mg/L,KNO_3 2000mg/L,KH_2LO_4 100mg/L)。在此基础上,讨论了工厂化组培繁殖木本植物特别是一些常规繁殖困难的林木树种的可能性,以及提高组培生产率和降低生产成本的有效措施。

This report recommended a revised MS basal medium (macroelements were modified as follows: NH4NO3 850 mg/L, KNO3 2000 mg/L, KH2PO4 100 mg/L, which is suitable for tissue culture of woody plants, by studying effects of three major nutrient elements (nitrogen, potassium and phosphorus) on the propagation in vitro of several woody plants (Cunninghamia lanceolata (Lamb.) Hook., Sequoia sempervirens (Lamb.) Lindl., etc.).Based on the data of the researches, the possibility in mass propagation in vitro of woody plants in particular those with difficulty for conventional propagation and some effective measures for increase of productivity and decrease of production cost were discussed.