蛋白改造提高苏云金杆菌杀虫活力的研究进展

Advances in Enhancing the Toxicity of Bacillus thuringiensis with Protein Modification

苏云金杆菌(Bt)已被广泛应用于农林害虫防治,但传统制剂仍然存在很大局限性,利用蛋白质工程对其改造能有效的克服这些缺点。主要概述了近年来利用结构域转换和定点突变技术改造BtCry蛋白并增强其杀虫活性的研究进展。

Bacillus thuringiensis（Bt） has been widely used as a biopesticide in agriculture, forestry and mosquito control, but wild Bt strains still have many disadvantages, which can be resolved by protein engineering of the Bt δ-endotoxins. In this review, the recent progress in enhancing the toxicity or broadening insecticidal spectrum of Bacillus thuringiensis by technologies such as swapping domains and site-directed mutagenesis,were summarized.