鹅细小病毒LH株的分离鉴定

Isolation and Identification of Goose Parvovirus Strain LH

采用鹅胚接种方法从南京六合具典型鹅细小病毒病病变的死亡雏鹅体内分离到1株鹅细小病毒(LH株)。在电镜下观察到该病毒液具细小病毒样粒子;8日龄健康易感雏鹅人工感染后100%发病、死亡,且具有典型鹅细小病毒病临床症状和剖检特征。在琼脂扩散试验中,用感染鹅胚液制备的待检沉淀抗原,能与鹅细小病毒阳性血清发生特异性反应。应用针对GPVVP3特异性引物对LH毒液进行PCR检测,扩增出的片段为1.6 kb,与目的条带大小相符。序列分析发现,扩增产物与已发表的GPV的VP3片段相比较,同源性达99%以上,表明所分离到的LH株是一株鹅细小病毒。

The strain LH of goose parvovirus （GPV） was isolated from the infected young goose in Liuhe District of Nanjing. The virus were propagated in goose embryos of 12-day-old, and showed typical shape of parvovirus under electronic microscope. The goose of 8-day-old were artificially inoculated with the virus sample, infection rate and mortality was 100% , and the clinic symptom and pathological lesion were similar to the natural infected cases of GPV. The GPV antigen from allantoie fluid of infected embryos could react with positive serum of GPV by agar gel precipitation. The strain LH liquid was detected by PCR with the specific primers aim at GPV VP3, and the result showed a bound of 1.6 kb, coincide with expected bound. Sequence analysis showed 99%-100% nueleotide homology between the amplified product and the other published GPV strains, which indicated the isolated strain LH was goose parvovirus.