电针不同组穴对兔腰椎脊髓损伤后瘫痪肢体皮下血流量的影响

Influence of Electroacupuncture at Different Groups of Points on Subcutaneous Blood Flow in the Paralyzed Limb of Rabbits with Lumbar Spinal Cord Injury

目的观察电针不同组穴对L1脊髓损伤后瘫痪肢体皮下血液灌流量的影响,比较两组穴的作用特点。方法用改良的ALLEN’S致伤法,造成L1脊髓不完全损伤模型,然后按不同组穴(督脉穴组、背俞穴组)进行电针治疗,同时设立对照组、空白组;应用激光多普勒血流仪和Powerlab十六道生理记录仪测定瘫痪家兔阴陵泉皮下血流量,观察时间点为损伤前20min、损伤后2h、第7天及第13天。结果L1脊髓损伤后2h瘫痪肢体皮下血流量有明显降低;损伤后第13天督脉穴组家兔肢体皮下血流量显著恢复,与对照组相比有统计学意义。结论督脉穴组对腰椎损伤后瘫痪肢体皮下血流量的恢复有积极作用,且优于背俞穴组。

Objective To observe the influence of electroacupuncture at different groups of points on subcutaneous blood flow in the paralyzed limb after L1 spinal cord injury and compare the characteristics of the actions of two groups of points. Methods A model of incomplete L1 spinal cord injury was made by the modified ALLEN＇s method of producing injury. Electroacupuncture was performed at different groups of points （Governor Vessel point group and Back-Shu point group）. A control group and a blank group were established at the same time. Subcutaneous blood flow at Yinlingquan （SP9） in the paralyzed rabbits was measured using a laser Doppler blood flowmeter and a Powerlab 16-channel data acquisition system at 20 min before and at 2 hrs, 7 days and 13 days after the injury. Results Subcutaneous blood flow in the paralyzed limb decreased obviously at 2 hrs after L1 spinal cord injury. Subcutaneous blood flow in the rabbit limb increased obviously in the Governor Vessel point group at 13 days after the injury compared with the Back-Shu point group. There was a statistically significant difference. Conclusion Governor Vessel points have a positive restoring effect on subcutaneous blood flow in the paralyzed limb after lumbar spinal cord injury. The effect is superior to that of Back-Shu points.