血管粘接吻合密封胶(剂)的实验研究

Study of Sealant Gels for Vascular Anastomosis with Adhesive, Cyanoacrylate Glue and Intravascular Soluble Hollow Stents

目的:研制一种血管粘接吻合密封胶(剂),可以保护吻合口,促进血管通畅。方法:参照化工学的理论和方法,进行实验研究;从海藻酸钠、壳聚糖、明胶等生物相溶性较好的物质中,依据反应成膜性能、膜的水溶解性和生物相溶性,以及自身流动性和表面接触角度等特点,先进行组分筛选,再行研制确定。结果:通过成膜性筛选实验,发现只有海藻酸钠符合要求,其与CaCl2反应能瞬间成膜,其膜不溶于水;通过测定其流动性和表面接触角度等,发现其质量分数为1%～3%的水溶液各种性能较好;其与质量分数为20%的CaCl2溶液反应成膜的生物相容性较好。结论:质量分数为1%～3%的海藻酸钠水溶液与质量分数为20%的CaCl2组成的双组分密封胶,生物实验中各项性能良好,在新的医用胶面世以前,可以作为血管粘接吻合密封剂,有望在临床中实验推广,为临床血管粘接吻合应用提供理论依据。

Objective To research and make one kind of sealant gel to prevent glue from leaking effectively,and protect vascular anastomosis site,raise the vascular anastomosis patency.Methods The experiment was carried out based on theory and methods in chemistry;According to the membrane performance,the membrane water solubility,and other characters,the author took experiment on the alginic acid sodium,chitsan,the gelatin and other biological materials.Results Through the experiment by membrane performance,the alginic acid sodium met the requirement, which became the membrane with the CaCl2 reaction at once. Its membrane was not dissolved in the water. And the characters of its 1%-3% solution were good on fluidity and surface contact angle determines. The biological compatibility of the compound of it with 20% CaCl2 solution was good. Conclusion The characters of the composition, which is 7：louble component sealant made from 1-3% alginic acid sodium and 20%CACl2 solution, is best in the biological experiment, Before new medical glue appeared in clinical, this gel is hopeful to be applied in clinic and provide theory basis for clinical anastomosis.