SLE患者外周血Th17细胞的表达及其临床意义

The Clinical Significance of Increased Expression of Peripheral Blood Th17 Cells in Patients with Systemic Lupus Erythematosis(SLE)

目的:探讨Th17细胞与系统性红斑狼疮(SLE)的发生及疾病活动性的相关性。方法:采用酶联免疫吸附法(ELISA)检测正常人和SLE患者外周血单个核细胞(PBMCs)刺激培养上清液中IL-17的水平,并用流式细胞术(FCM)分析PBMCs中Th17细胞的频率。结果:与正常对照组相比,稳定期和活动期SLE患者PBMCs刺激后产生IL-17的量明显增高(P<0.01),Th17细胞的频率也高于正常对照组(P<0.01)。与静止期患者相比,活动期SLE患者PBMCs产生IL-17的水平和Th17细胞的频率也明显增高(P<0.05)。结论:Th17细胞可能通过分泌细胞因子IL-17参与了SLE发病及病情发展,其水平高低部分反映SLE疾病的活动性。

Objective To study the relationship between increased expression of peripheral blood Th17 cells and the activity of the disease in patients with SLE.Methods Concentration of IL-17 and expression of Th17 cells in the PBMCs culture supernatant（stimulated with anti-CD3 and anti-CD28 mAb） were determined with ELISA and flows-cytometry respectively in 45 patients with SLE（active stage,n=20,inactive or stable stage,n=25） and 40 controls.Results The Th17 cells expression and IL-17 concentration in the stimulated PBMCs culture supernatant were significantly higher in the patients with SLE as a whole than those in controls（P〈0.01）.Among the SLE patients,the Th17 expression and IL-17 concentration in the patients in active stage were significantly higher than those in patients within stable stage（P〈0.05）.Conclusion Th17 cells might play important role in the activation of the disease through increased secretion of IL-17 in patients with SLE.