五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤

Synergistic protective effect of Fructus Schisandrae chinensis with Astragalus polysaccharides on paracetamol-induced acute liver injury in mice

目的:研究五味子提取物和黄芪多糖配伍后对对乙酰氨基酚引起小鼠急性肝损伤的协同保护作用及其机制。方法:以对乙酰氨基酚500 mg/kg腹腔注射给药造成小鼠急性肝损伤模型,通过测定小鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)的活性、肝组织还原性谷胱甘肽(GSH)和丙二醛(MDA)含量及观察肝组织病理学改变,以评价五味子提取物、黄芪多糖及其组合物对小鼠肝损伤的保护作用。结果:五味子提取物(270 mg/kg)和黄芪多糖(900 mg/kg)单独给药对对乙酰氨基酚肝损伤小鼠的血清ALT、AST和肝组织GSH和MDA没有显著性影响。30、90和270 mg/kg五味子提取物分别与100、300和900 mg/kg黄芪多糖配伍,可使对乙酰氨基酚肝损伤小鼠血清ALT、AST活性及肝组织MDA含量显著降低(P<0.05或P<0.01),肝组织GSH含量显著提高(P<0.05或P<0.01),肝组织细胞的病变程度得到明显改善。在高剂量配伍下,各指标的五味子提取物和黄芪多糖间相互作用指数CDI均小于0.7。结论:五味子提取物和黄芪多糖通过协同提高对乙酰氨基酚致肝损伤小鼠肝脏的还原性谷胱甘肽和抗氧化水平,降低血清ALT和AST水平,减轻肝组织细胞的损伤。

Objective： To study the synergistic protective effect and mechanism of extracts from Fructus Sehisandrae chinensis （EFS） with astragalus polysaccharides （APS） on paracetamol-indueed liver injury in mice. Methods： Mice liver injury was induced by peritoneal injection paracetamol physiologic saline solution at a dose of 500 mg/kg. The activities of serum alanine aminotransferase （ALT） and asparate aminotransferase （AST） , the contents of malondialdehyde （MDA） and glutathione （GSH） in the liver tissue, and the liver histopathological changes were observed to evaluate the effects of EFS, APS and their combination on liver injury. Results： The EFS （270 mg/kg） or APS （900 mg/kg） alone did not produce any effect on liver injury induced by paracetamol in mice. The combination of EFS at dose of 30,90 and 270 mg/kg respectively with APS at dose of 100,300 and 900 mg/kg significantly decreased the activities of ALT and AST in serum and MAD level in liver tissue （P 〈0.05 or P 〈0.01 ）, increased the GSH content in liver tissue （P 〈 0.05 or P 〈 0.01 ） , and improved liver histopathological changes. The coefficients drag interaction （CDI） of related parameters between EFS （270 mg/kg） and APS （900 mg/kg） were all less than 0. 7. Conclusion：The combination of EFS and APS reduced the ALT and AST level in serum, improved the liver histopathological changes of liver injury mice through synergistically in- creasing GSH eontent and antioxidant ability of liver.