HIP1在维生素K3诱导Hela细胞损伤中的表达及意义

Expression of HIP1 in injuried Hela cells induced by vitamin K3 and significance

目的:观察Huntingtin相关蛋白1(HIP1)在维生素K3诱导Hela细胞损伤中的表达及意义。方法:对数生长期Hela细胞随机分为对照组(Control)、VK3处理组(VK3)、VK3与NAC联合作用组(VK3+NAC)及NAC单独应用组(NAC);MTT法检测各组细胞存活率;Western blotting法检测各组HIP1、NFκ-B蛋白表达水平;RT-PCR方法检测各组Cyclin D1 mRNA表达水平。结果:与对照组相比,VK3组Hela细胞存活率降低(P<0.01),HIP1蛋白、NF-κB蛋白表达量降低(P<0.05),Cyclin D1 mRNA表达量明显下降(P<0.05);与VK3组相比,VK3与NAC联合应用组细胞存活率增加,能够明显拮抗HIP1蛋白,NFκ-B蛋白表达量和Cyclin D1 mRNA表达量均增加(P<0.05)。结论:VK3具有抑制细胞增殖作用,可能与降低HIP1的蛋白表达水平有关。

Objective To observe the expression of Humtingtin interacting protein 1（HIP1） in the injury of Hela cells induced by vitamin K3（VK3）.Methods Hela cells in log phase were randomly divided into control group,VK3 group,VK3＋NAC group and NAC group,respectively.The survival rate of Hela cells was determined by MTT.The expressions of HIP1 and NF-κB protein in Hela cells were detected by Western blotting.The expression of Cyclin D1 mRNA was tested by RT-PCR assay.Results Compared with control group,the survival rate of Hela cells in VK3 group decreased（P〈0.05）;the expressions of HIP1 and NF-κB protein and Cyclin D1 mRNA were all decreased in VK3 group（P〈0.05）.Compared with VK3 group,the survival rate of Hela cells in VK3 ＋ NAC group was increased（P〈0.05）;the expressions of HIP1 and NF-κB protein and Cyclin D1 mRNA were increased（P〈0.05）.Conclusion VK3 has an effect of inhibiting the cell proliferation which may be related with down-regulation of HIP1 protein expression.