嘌呤核苷酸补偿对海洛因依赖大鼠嘌呤核苷酸分解代谢的影响

Effects of purine nucleotide compensation on purine nucleotide catabolism in heroin dependent rats

目的:探讨外源性补偿嘌呤核苷酸对海洛因依赖大鼠嘌呤核苷酸分解代谢的影响,阐明嘌呤核苷酸补偿治疗海洛因依赖效应机制。方法:将50只雄性Wistar大鼠随机分为对照组、海洛因组、海洛因+AMP+GMP组、海洛因+AMP组、海洛因+GMP组。检测各组血尿酸(UA)、尿素氮(BUN)和肌酐(Cre)含量,以及血浆和大脑皮质腺苷脱氨酶(ADA)和黄嘌呤氧化酶(XO)活性。结果:海洛因组血浆中UA含量及ADA、XO的活性均比对照组明显升高(P<0.05),而海洛因+AMP+GMP组、海洛因+AMP组、海洛因+GMP组血浆UA含量及ADA、XO的活性与海洛因组相比明显降低(P<0.05)。各组间BUN和Cre含量差异无显著性(P>0.05)。海洛因组大脑皮质中ADA、XO的活性均比对照组明显升高(P<0.05),而海洛因+AMP+GMP组、海洛因+AMP组、海洛因+GMP组大脑皮质ADA、XO的活性与海洛因组相比明显降低(P<0.05)。结论:海洛因可促进大鼠整体水平上嘌呤核苷酸的分解代谢,而补偿嘌呤核苷酸可以抑制或拮抗海洛因的上述作用。

Objective To explore the effect of exogenous purine nucleotide on the catabolism of nucleotide in heroin dependent rats,and clarify the mechanism of purine nucleotide treatment for heroin dependent rats.Methods Fifty male Wistar rats were randomly divided into control group,heroin administration group,heroin plus AMP and GMP group,heroin plus AMP group and heroin plus GMP group.The contents of uric acid（UA）,blood urea nitrogen（BUN） and creatinine（Cre） in plasma were measured,the activities of adenosine deaminase（ADA） and xanthine oxidase（XO） in plasma and brain cortex were measured by test Kits.Results The content of UA in plasma and the activities of ADA,XO in heroin group were significantly higher than those in control group and three heroin plus purine nucleotides groups（P〈0.05）.The contents of BUN and Cre had no difference between various groups（P〉0.05）.The ADA and XO activities in plasma and brain cortex in heroin group were significantly increased compared with control group and three heroin plus purine nucleotides groups（P〈0.05,P〈 0.01）.Conclusion Compensating purine nucleotides could ameliorate the enhancement of purine nucleotide catabolism.