盐酸多奈哌齐对未成年大鼠学习记忆功能的影响

Effect of donepezil hydrochloride on learning and memory function of normal under age rats

目的：探讨盐酸多奈哌齐对未成年大鼠学习记忆功能的影响,为盐酸多奈哌齐的临床应用提供实验依据。方法：选用刚离乳的40只Wistar大鼠,分为正常对照组及盐酸多奈哌齐组,每组20只盐酸多奈哌齐0.45 mg·kg^-1,连续给药8周,通过Morris水迷宫、跳台行为学实验,观察盐酸多奈哌齐对正常未成年大鼠空间分辨能力和被动回避能力的影响。结果：与对照组比较,盐酸多奈哌齐大鼠第1-6天达到平台的潜伏期、游程、平均速度、朝向角及第7天大鼠在2 min内的穿越平台的次数、在平台区的逗留时间、平台象限内的逗留时间、平均速度及平台区象限内的游程占总游程的百分比均无明显变化（P〉0.05）;大鼠第1和2天跳台的错误次数无明显减少,第2天错误的潜伏期无明显延长（P〉0.05）。结论：盐酸多奈哌齐对正常未成年大鼠学习记忆功能无促进作用。

Objective To explore the effect of donepezil hydrochloride on learning and memory function of normal under age rats,and provide experimental basis for clinical application of donepezil hydrochloride.Methods Fourty Wistar rats just away from galactic were divided into control group（n=20） and donepezil hydrochlorid group（n=20）,donepezil hydrochloride was given to the rats in donepezil hydrochloride group（0.45 mg·kg-1,8 weeks）,the effects of donepezil hydrochloride on space discrimination ability and passive avoidance ability of normal under age rats were detected with Morris water maze and Step-down behavior test.Results Compared with control group,the latency,distance,average speed and starting angle of rats treated by donepezil hydrochloride did not change significantly from the first day to the sixth day,the time in platform,time in platform quadrant,times passing platform,distance in platform / total distance×100% and average speed also did not change significantly in the seventh day in the Morris water maze（P〉0.05）;the error times of rats treated by donepezil hydrochloride did not decrease in the first day and in the second day,the latency did not prolong in the second day in Step-down behavior test（P〉0.05）.Conclusion Donepezil hydrochloride do not improve the learning and memory function of normal under age rats.