骨髓间充质干细胞移植对大鼠肝纤维化模型的修复作用

Repairment of marrow derived mesenchymal stem cells on hepatic fibrosis in rats

目的:观察骨髓间充质干细胞(MSCs)移植对大鼠肝纤维化模型的修复作用,为MSCs的临床应用提供可靠的理论依据。方法:分离、纯化大鼠MSCs并进行体外培养。用皮下多点注射CCl4制备肝纤维化大鼠模型,将大鼠随机分为3组:正常对照组、模型损伤组和MSCs移植组,每组6只。将MSCs经尾静脉回输到MSCs移植组大鼠体内,30 d取肝脏,观察病理变化,免疫组织化学检测α平滑肌肌动蛋白(α-SMA)的表达。结果:流式细胞术检测体外分离培养的MSCs 93.27%处于G0/G1期,且MSCs表面CD44呈阳性表达;将分离的MSCs输入肝纤维化模型大鼠30 d后,镜下可见MSCs移植组病理改变较模型损伤组明显减轻,肝小叶结构基本正常,肝血窦基本正常,纤维结缔组织无明显增生;α-SMA染色强度MSCs移植组低于模型损伤组。结论:MSCs在一定程度上可以促进纤维化肝组织的修复。

Objective To observe the repairing effect of marrow derived mesenchymal stem cells（MSCs） on hepatic fibrosis in rats,and provide a reliable theoretical basis for further clinical application of MSCs.Methods The MSCs of rats were isolated,purified and cultivated in vitro.The model of hepatic fibrosis induced by CCl4 was established.The rats were divided into three groups：normal control group,model group,MSCs transplantation group,6 each group.The MSCs were transplatated into the rats through tail vein in MSCs transplantation group.The repairing effect of MSCs on hepatic fibrosis was observed and the expression of α-SMA in liver tissue was detected by immunohistochemistry 30 d after transplantation.Results 93.27% MSCs were at G0/G1 phase,and the CD44 surface expression in the MSCs was positive.The hepatic pathological injury changes of rats in MSCs transplantation group were significantly reduced than that in model group 30 d after MSCs injection.The lobular structure and liver sinusoids were normal and hyperplasia of fibrous connective tissue was not obvious.The intensity of α-SMA staining in MSCs transplantation group was lower than that in model group.Conclusion MSCs have repairment on hepatic fibrosis in rats to a certain extent.