沙蚕消化道D2菌株胞外蛋白酶对EMT6细胞增殖的影响

Effect of protease from strain D2 in digestive tract of Perinereis aibuhitensis Grube on the proliferation of EMT6 cells

目的研究沙蚕消化道产蛋白酶菌D2株胞外蛋白酶对鼠乳腺癌细胞EMT6增殖的影响。方法选用鼠乳腺癌细胞EMT6作为靶细胞，用0．0043～43μmol／L浓度的沙蚕消化道产蛋白酶菌D2株胞外蛋白酶（D2蛋白酶），通过体外细胞培养法进行细胞增殖抑制检测，测其吸光度（OD）值，计算细胞增殖抑制率，与阳性对照组（紫杉醇组）比较。结果实验组OD值低于空白对照组，且存在时间和剂量依赖关系。结论沙蚕消化道产蛋白酶菌D2株胞外蛋白酶对鼠乳腺癌细胞EMT6的增殖存在抑制作用，显示出一定的抗肿瘤活性。

Objective To determine the effect of protease from D2 in digestive tract of Perinereis aibuhitensis Grube on the proliferation of EMT6 cells. Methods EMT6 cells were cultured in the presence of protease at gradient concentrations of 0.0043-43μmol/L for different time. The inhibition of cell proliferation was evaluated by MTT assay and compared with positive control group. Results The D2-derived protease can decrease the viability and inhibit the proliferation of EMT6 cells in dose-dependent and time-dependent manners. Conclusions Protease may have anti-neoplastic activity in some degree.