固定化酵母催化的ATP生产与CO_2释放率的关系研究

STUDY ON RELATIONSHIP BETWEEN ATP PRODUCTION WITH IMMOBILIZED SACCHARAMYCES CEREVISIAE AND CO_2 EVOLUTION RATE

用Ｙ－Ｚ型多功能摇瓶，考察了固定化酵母转化腺苷生产三磷腺苷（ＡＴＰ）过程中ＣＯ２释放率（ＣＥＲ）与葡萄糖、腺苷、腺苷二磷酸、磷酸腺苷在反应液中的积累情况。结果表明，ＣＥＲ在整个反应过程中可分为缓慢上升、快速上升和下降３阶段。根据ＣＥＲ的变化可很快估算出葡萄糖的消耗状况，分析判断出反应进程。

The relationship between CO2 evolution rate(CER) and other parameters such as the consumption of glucose and Ado, the accumulation of ATP, ADP and AMP in the ATP production process catalyzed by immobilized Saccharamyces Cerevisiae in a YZ type multifunction shaking flask were discussed systematically. The result showed that the CER curve of whole process could be divided into three stages of slow increasing, rapid increasing and decreasing. The ATP production and glucose consumption might be estimated, so that the process of the reaction could be online controlled according to CER.