不同负荷游泳训练对脑缺血再灌注大鼠Caspase-3和IL-1β的表达影响

Exercise Training on Expression of Caspase-3 and IL-1β in Rats with Focal Cerebral Ischemia-reperfusion

目的研究运动训练对局部脑缺血再灌注大鼠Caspase-3和IL-1β的表达影响。方法将70只大鼠随机分为假手术组、缺血再灌注模型组、再灌注+10min训练组及缺血再灌注+30min训练组,采用线栓法制作SD大鼠大脑中动脉闭塞(MCAO)再灌注模型。运动组大鼠术后每天进行游泳训练,6周后观测每组Caspase-3和IL-1β的指标。结果大鼠局部脑缺血再灌注后,每天30min运动训练的大鼠脑组织Caspase-3表达量低于缺血再灌注模型组(P<0.05),同时,IL-1β量也下降(P<0.05)。结论合理的运动训练能促进缺血再灌注大鼠损伤部位的恢复,其相关机制可能与减轻局部有害因子的表达有关。

Objective To study the effect of exercise training on the expression of Caspase-3 and IL-1β in rats with focal cerebral ischemia-reperfusion.Methods Seventy rats were recruited in this study and randomly divided into 4 groups：sham operation group,ischemic reperfusion group,ischemic reperfusion group with 10 min exercise per day,and ischemic reperfusion group with 30 min exercise per day.The exercise training was swimming training daily after the operation.Results Six months after training the Caspase-3 and IL-1β in ischemic cerebral tissue with 30 min was lower than that in ischemic reperfusion group（P〈0.05）.Conclusion Exercise training may improve the functional recovery of extremities in rats with cerebral ischemia-reperfusion,and the mechanism was related to decrease the expression of poisonous factors.