基因芯片技术分析抗癌生物活性肽对人胆管癌QBC939细胞凋亡基因表达谱调控的影响

Gene chips are used for studying how anticarcinoma bioactive peptides influence the expression of cholangiocarcinoma QBC939 apoptotis gene

目的利用基因芯片探讨抗癌活性肽诱导人胆管癌细胞QBC939细胞后凋亡相关基因表达的差异性;寻找关键基因并分析其可能的作用机制。方法采用RPMI1640培养基体外培养人胆管癌QBC939细胞,将其分为两组,空白对照组和抗癌活性肽实验组,选用指数增长期细胞用于实验。Trizol法提取总RNA,采用定制基因芯片对1 153条凋亡相关基因的表达进行检测,对基因芯片数据进行处理和生物学信息分析。结果在1 153条基因中,抗癌活性肽组与对照组比较基因表达谱存在差异;差异表达基因中,上调基因121条,下调基因120条,其中表达差别有显著性意义的基因共89条,包括下调基因84条,上调基因4条,无变化1条。上调显著基因为共济失调毛细血管扩张症突变(ATM)基因、3-磷酸甘油醛脱氢酶(GAPDH)基因。结论抗癌活性肽对多种胆管癌细胞凋亡相关基因有调控作用,研究结果为进一步开展抗癌活性肽调控靶基因的研究奠定了基础。

Objective To investigate the gene expression differences of anti cancer bioactive peptide-induced QBC939 cells using gene chip and search for the key genes and the relative mechanism.Methods Cholangiocarcinoma QBC939 cell was cultured using RPMI1640 medium.Cells were divided into control and anti cancer bioactive peptide groups.Exponential increasing cells were used.RNA was abstracted using TRIZOL reagent and microarray containing 1 153 pieces of apoptosis related genes was applied.Data was processed and analyzed using bioinformatics knowledge.Results Gene expression differences were found in peptide and control groups among 1 153 genes.121 genes were up-regulated and 120 genes were down-regulated,There were totally 89 differentially expressed genes on the two chips,of which 4 were up-regulated and 84 were down-regulated.in which,most markedly up-regulated genes included ataxia-telangiectasia mutated gene,ATM and GAPDH.Conclusions Anti cancer bioactive peptide can regulate apoptosis re lated genes during the occurrence and development of cholangiocarcinoma.Our results provided the research basis of the regulated target genes of anti cancer bioactive peptide.