摘要
以马铃薯克新4号试管苗的离体茎尖为试材,通过正交设计试验对预培养培养基中蔗糖浓度、预培养时间和PVS2处理时间等影响超低温保存存活率的主要因素进行了分析,初步建立了马铃薯种质玻璃化超低温保存的技术方案。通过形态观察和同工酶检测,冻存前后材料的遗传稳定性没有发生改变,表明该方法对马铃薯的种质保存具有较强的实用意义。
Shoot tips from in vitro plants of the potato cuhivar Kexin No.4 were cryopreserved by vitrification method. Orthogonal design was used to analyze the effects of dominant parameters on survival rates of the shoot tips, including sucrose concentration of preculture medium, duration of preculture and duration of exposure to PVS2. There were no variations in the regenerated plants for morphology and isoenzyme zymogram after cryopreservation. This vitrification method appears promising for cryopreservation of the in vitro potato germplasm resources.
出处
《中国马铃薯》
2009年第5期268-270,共3页
Chinese Potato Journal
基金
国家科技支撑计划"农作物基因资源安全保存评价关键技术研究"(2006BAD13B10-05)
关键词
马铃薯种质资源
正交设计
超低温保存
potato germplasm resources
orthogonal test
cryopreservation
