体外膀胱癌细胞光动力学杀伤效应的研究

In vitro photodynamic therapy of the human bladder cancer cells

目的：探索叶绿素衍生物（ＣＰＤ４）光动力学疗法在体外人膀胱癌细胞的杀伤效应中不同的激光波长、能量和ＣＰＤ４浓度之间的关系。方法：采用ＭＴＴ比色分析法判别体外人膀胱癌Ｔ－２４和ＳＣａＢＥＲ细胞对不同的激光波长、能量和ＣＰＤ４浓度的光敏效应。结果：ＣＰＤ４的光敏效应与激光能量和药物浓度呈正相关，并在相同激光能量和药物浓度的情况下，绿光（５１０ｎｍ）的光敏效应明显强于红光（６３０ｎｍ）。结论：ＣＰＤ４光动力学作用对体外人膀胱细胞癌有明显杀伤效应，而绿光明显强于红光。

Objective:The efficacy of laser wavelength, energy and CPD 4 concentration was studied in vitro photodynamic therapy(PDT) of the human bladder cancer cells.Method:The photodynamic cytotoxicity of human bladder cancer cells(T-24 and SCaBER) was studied by MTT rapid colormetric assay referring to different CPD 4 concentration,laser energy and wavelength.Results:The photodynamic cytotoxicity of the CPD 4 was directly correlated to the laser energy and concentration of photosensitizer.Under identical laser energy and CPD 4 concentration conditions,the photodynamic cytotoxicity of green light(wavelenght,510nm) was significantly stronger than red light(wavelength,630nm).Conclusions:CPD 4-PDT is effective for killing the human bladder cancer cells in vitro.Green light is stronger than the red light in photodynamic cytotoxicity.These data provide the basis in clinical photodynamic therapy of early lesions of bladder cancer and in preventing postoperative recurrences.The green light is preferable to the red light.