摘要
目的探讨急性早幼粒细胞白血病细胞抗原表达的情况。方法采用三色荧光标记流式细胞仪检测33例FAB分型为AML-M3患者的骨髓。结果AML-M3患者CD45/SSC双参数散点图有其独特性,通常只可见两群细胞,其中正常淋巴细胞群比例减少,而幼稚细胞群所占比例增高且其SSC值较高,本研究中平均达90%以上。33例M3全部表达CD13、CD33,阳性率为100%,33例中2例分别表达CD34和HLA-DR,其表达阳性率各为3.0%,CD117表达百分比为63.6%,支持CD117在APL患者原始细胞表达较高水平的观点。结论三色荧光标记流式细胞术,CD45/SSC设门方法可将幼稚细胞与正常细胞区分开来,可特异地对幼稚细胞进行分析,极大地提高了APL免疫学分型的诊断准确率。
Objective To explore the immunophenoytpe of acute promyelocytic leukemia. Methods Immunophenotype was examined by three-color flow eytometry in bone marrow of 31 patients with AML-M3 of FAB. Results CD13 and CD33 were expressed in all 33 patients（100% ）. CD34 and HLA-DR were expressed in 2 of 33 （3.0%） and CD117 were expressed in 21 of 33 （63.6%）. Conclusion CD45/SSC door in three-color flow cytometry can classify effectively naive cells from normal cells and specifically analyze he naive cell. This method can improve diagnositic accuracy of immunophenoytpe in acute promyelocytic leukemia.
出处
《宁夏医科大学学报》
2009年第5期590-592,F0002,共4页
Journal of Ningxia Medical University
